RRC ID |
42817
|
著者 |
Yamashita R, Fujiwara Y, Ikari K, Hamada K, Otomo A, Yasuda K, Noda M, Kaburagi Y.
|
タイトル |
Extracellular proteome of human hepatoma cell, HepG2 analyzed using two-dimensional liquid chromatography coupled with tandem mass spectrometry.
|
ジャーナル |
Mol Cell Biochem
|
Abstract |
Secreted proteins, which may be involved in the regulation of various biological processes, are the potential targets for diagnosis and treatment of diverse diseases. In this study, to identify the human hepatoma HepG2 cells-derived secreted proteins more extensively, we applied the protein sample preparations using the combinations of denaturation methods and molecular-mass cutoff via ultrafiltration to the two-dimensional liquid chromatography coupled with tandem mass spectrometry (2D LC-MS/MS) analysis. We were able to identify a total of 86 proteins containing widely known secreted proteins of HepG2 such as alpha-fetoprotein, of which 73 proteins including 27 signal peptide-containing proteins have never been reported to be secreted from HepG2 cells in other proteomic studies. Among the identified signal peptide-containing proteins, ten proteins such as growth differentiation factor 15, osteopontin and stanniocalcin 2 were discovered as new secreted proteins of HepG2 cells. These observations suggest that the combinations of different sample preparation methods and 2D LC-MS/MS analysis are useful for identifying a wider range of low-abundance proteins and that the secreted proteins from HepG2 identified in this study may be useful as liver-specific biomarkers for diagnosis and treatment.
|
巻・号 |
298(1-2)
|
ページ |
83-92
|
公開日 |
2007-4-1
|
DOI |
10.1007/s11010-006-9354-9
|
PMID |
17109077
|
MeSH |
Carcinoma, Hepatocellular / chemistry*
Carcinoma, Hepatocellular / metabolism
Cell Line, Tumor
Chromatography, Liquid
Humans
Liver Neoplasms / chemistry*
Liver Neoplasms / metabolism
Neoplasm Proteins / chemistry
Neoplasm Proteins / metabolism
Proteome / analysis*
Tandem Mass Spectrometry / methods*
|
IF |
2.795
|
引用数 |
34
|
WOS 分野
|
CELL BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
Hep G2 |