| RRC ID |
42823
|
| 著者 |
Watanabe N, Hirayama R, Kubota N.
|
| タイトル |
The chemopreventive flavonoid apigenin confers radiosensitizing effect in human tumor cells grown as monolayers and spheroids.
|
| ジャーナル |
J Radiat Res
|
| Abstract |
Apigenin, a common dietary flavonoid present in many fruits and vegetables, is a nonmutagenic chemopreventive agent. In the present study, we investigated the effect of apigenin on the radiosensitivity of SQ-5 cells, which are derived from a human lung carcinoma. Actively growing cells were incubated for 16 h at 37 degrees C in medium containing 40 muM apigenin. The cells were then irradiated with X-rays and incubated with apigenin for a further 8 h. Radiosensitivity was assessed using a clonogenic assay. Apoptosis and necrosis were assessed using acridine orange/ethidium bromide double staining. Cells incubated with apigenin exhibited significantly greater radiosensitivity and apoptosis levels than cells not incubated with apigenin. Protein levels were measured by Western blotting. Incubation with apigenin increased protein expression of WAF1/p21 and decreased protein expression of Bcl-2. Furthermore, apigenin sensitized SQ-5 spheroids (cell aggregates growing in a three-dimensional structure that simulate the growth and microenvironmental conditions of in vivo tumors) to radiation. Thus, apigenin appears to be a promising radiosensitizing agent for use against human carcinomas.
|
| 巻・号 |
48(1)
|
| ページ |
45-50
|
| 公開日 |
2007-1-1
|
| DOI |
10.1269/jrr.0635
|
| PII |
JST.JSTAGE/jrr/0635
|
| PMID |
17132915
|
| MeSH |
Anticarcinogenic Agents / administration & dosage
Apigenin / administration & dosage*
Cell Line, Tumor
Cell Survival / drug effects*
Cell Survival / radiation effects*
Dose-Response Relationship, Drug
Dose-Response Relationship, Radiation
Humans
Lung Neoplasms / pathology*
Radiation Dosage
Radiation Tolerance / drug effects*
Spheroids, Cellular / pathology
Spheroids, Cellular / radiation effects
|
| IF |
1.95
|
| 引用数 |
18
|
|
WOS 分野
|
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
