| RRC ID |
42834
|
| Author |
Saitoh H, Okumura S, Ishikawa T, Akao T, Mizuki E, Ohba M.
|
| Title |
Investigation of a novel Bacillus thuringiensis gene encoding a parasporal protein, parasporin-4, that preferentially kills human leukemic T cells.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
A novel gene encoding a leukemic cell-killing parasporal protein, designated parasporin-4, was cloned from an isolate of Bacillus thuringiensis serovar shandongiensis. The amino acid sequence of the parasporin-4, as deduced from the gene sequence, had low-level homologies of <30% with the established B. thuringiensis Cry proteins including the three known parasporins. When the gene was expressed in a recombinant of Escherichia coli BL21(DE3), the parasporin-4 formed intracellular inclusion bodies. Alkali-solubilized and proteinase K-activated inclusion protein exhibited strong cytotoxic activity against human leukemic T cells (MOLT-4) and weak for normal T cells, but no adverse effect on human uterus cervix cancer cells (HeLa).
|
| Volume |
70(12)
|
| Pages |
2935-41
|
| Published |
2006-12-1
|
| DOI |
10.1271/bbb.60352
|
| PII |
JST.JSTAGE/bbb/60352
|
| PMID |
17151476
|
| MeSH |
Bacillus thuringiensis / genetics*
Base Sequence
Cell Death / drug effects*
Cell Line, Tumor
DNA Primers
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Endotoxins / genetics*
Endotoxins / pharmacology
Genes, Bacterial*
Humans
Leukemia / pathology*
Molecular Sequence Data
T-Lymphocytes / drug effects
|
| IF |
1.516
|
| Times Cited |
18
|
|
WOS Category
|
CHEMISTRY, APPLIED
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
MOLT-4(RCB0206) |
