Reference - Detail
| RRC ID | 42838 |
|---|---|
| Author | Onoue S, Liu B, Nemoto Y, Hirose M, Yajima T. |
| Title | Chemical synthesis and application of C-terminally 5-carboxyfluorescein-labelled thymopentin as a fluorescent probe for thymopoietin receptor. |
| Journal | Anal Sci |
| Abstract |
Thymopentin (TP5) is a synthetic pentapeptide fragment, which corresponds to position 32 - 36 of thymic polypeptide thymopoietin. Thymopoietin and TP5 display a variety of biological functions, including phenotypic differentiation of T cells and the regulation of immune systems. Previous chemical modification experiments suggested that there was an absolute requirement for N-terminal amino acids to maintain the biological activity of TP5. On the basis of this structure-activity relationship, we designed and synthesized the C-terminally 5-carboxyfluorescein-coupled TP5 (TP5-FAM) as a fluorescent probe for thymopoietin receptor. TP5-FAM could bind to the membrane of human lymphoid cell lines, MOLT-4 cells, in which the thymopoietin receptor is expressed. The binding is specific and saturable (K(d) = 33 microM). TP5 and human splenopentin are nearly equipotent inhibitors of TP5-FAM binding to the thymopoietin receptor, but porcine secretin did not show any significant inhibition of TP5-FAM binding to MOLT-4 cells. Thus, TP5-FAM is suggested to be a potent and biologically active ligand that would be useful for studying the binding and functional characteristics of the human thymopoietin receptor. |
| Volume | 22(12) |
| Pages | 1531-5 |
| Published | 2006-12-1 |
| DOI | 10.2116/analsci.22.1531 |
| PII | JST.JSTAGE/analsci/22.1531 |
| PMID | 17159311 |
| MeSH | Animals Cell Line, Tumor Fluoresceins Fluorescent Dyes Humans Ligands Peptide Fragments / pharmacology Receptors, Peptide / analysis* Receptors, Peptide / antagonists & inhibitors Receptors, Peptide / chemistry Secretin / pharmacology Structure-Activity Relationship Swine Thymopentin* / chemistry Thymopentin* / pharmacology Thymopoietins / pharmacology |
| IF | 2.049 |
| Times Cited | 6 |
| WOS Category | CHEMISTRY, ANALYTICAL |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MOLT-4(RCB0206) |