論文 - 詳細
| RRC ID | 42843 |
|---|---|
| 著者 | Amemori S, Ootani A, Aoki S, Fujise T, Shimoda R, Kakimoto T, Shiraishi R, Sakata Y, Tsunada S, Iwakiri R, Fujimoto K. |
| タイトル | Adipocytes and preadipocytes promote the proliferation of colon cancer cells in vitro. |
| ジャーナル | Am J Physiol Gastrointest Liver Physiol |
| Abstract |
Obesity, a risk factor for colon cancer, is associated with elevated serum levels of leptin, a protein produced by adipocytes. The aim of the present study was to clarify the effects of adipose tissue on colon cancer proliferation by using cultured cell lines. To achieve this, colon cancer cells (CACO-2, T84, and HT29) were cocultured with adipose tissue, isolated mature adipocytes, and isolated preadipocytes in a three-dimensional collagen gel culture system. The adipocytes and preadipocytes used were isolated from C57BL/6J and leptin-deficient ob/ob mice. Proliferation of the cancer cells was evaluated by nuclear bromodeoxyuridine uptake. The adipose tissue, mature adipocytes, and preadipocytes isolated from C57BL/6J mice significantly increased the proliferation of the colon cancer cells. This trophic effect of mature adipocytes on the cancer cell lines was observed only for cells from lean littermates and not for those from ob/ob mice. In contrast, the trophic effect of preadipocytes was not abolished in ob/ob mice, and this finding was supported by the result that leptin had a trophic effect on cancer cells. In conclusion, adipocytes were able to enhance the proliferation of colon cancer cells in vitro, partly via leptin, suggesting that adipose tissues, including mature adipocytes and preadipocytes, may promote the growth of colorectal cancer. |
| 巻・号 | 292(3) |
| ページ | G923-9 |
| 公開日 | 2007-3-1 |
| DOI | 10.1152/ajpgi.00145.2006 |
| PII | 00145.2006 |
| PMID | 17170030 |
| MeSH | Adipocytes, White / cytology* Adipocytes, White / metabolism Adipose Tissue / cytology Adipose Tissue / metabolism Animals Caco-2 Cells Cell Line, Tumor Cell Proliferation* Coculture Techniques / methods Collagen / chemistry Colonic Neoplasms / pathology Gels HT29 Cells Humans Leptin / deficiency Leptin / genetics Mice Mice, Inbred C57BL Mice, Knockout Mice, Obese Receptors, Cell Surface / analysis Receptors, Leptin Stromal Cells / pathology |
| IF | 3.725 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Clinical guideline |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |