論文 - 詳細
| RRC ID | 42847 |
|---|---|
| 著者 | Tachibana A, Nishikawa Y, Nishino M, Kaneko S, Tanabe T, Yamauchi K. |
| タイトル | Modified keratin sponge: binding of bone morphogenetic protein-2 and osteoblast differentiation. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
A keratin sponge was chemically modified to obtain carboxyl and amino sponges by the alkylation of a large amount of active SH group on keratin proteins with iodoacetic acid and 2-bromoethylamine, respectively. The carboxyl sponge having a large amount of carboxyl group was a scaffold that could bind significant amounts of basic bioactive proteins, such as lysozyme and bone morphogenetic protein (BMP)-2, and drugs. Lysozyme (maximum 3.7 mg), a model of basic cytokines such as BMP-2, was absorbed by the carboxyl sponge (4.8 mg), but not by the amino sponge. The lysozyme was rapidly released from the carboxyl sponge in a buffer containing at a concentration higher than 0.5 M, but at 0.15 M, near the physiological ionic strength, after initial burst (only 11%), no significant release was observed (15%, 48 h). BMP-2 also bound the carboxyl sponge. The preosteoblast cells grown inside the BMP-2-loaded sponge differentiated, whereas those grown outside the sponge did not, suggesting that no significant amount of BMP-2 leaked into the surrounding media. The effects of BMP-2 were confined inside modified keratin sponge. Therefore, using in vivo, we expect that only internal osteogenesis will be induced and that no external heterotopis ossification will be induced. |
| 巻・号 | 102(5) |
| ページ | 425-9 |
| 公開日 | 2006-11-1 |
| DOI | 10.1263/jbb.102.425 |
| PII | S1389-1723(06)70685-5 |
| PMID | 17189169 |
| MeSH | Alkaline Phosphatase / metabolism Animals Biocompatible Materials / chemistry* Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins / chemistry Bone Morphogenetic Proteins / metabolism* Cell Differentiation Cell Line Keratins / chemistry Keratins / metabolism* Mice Muramidase / metabolism Osteoblasts / cytology* Osteogenesis Transforming Growth Factor beta / chemistry Transforming Growth Factor beta / metabolism* |
| IF | 2.366 |
| 引用数 | 57 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |