論文 - 詳細
| RRC ID | 42852 |
|---|---|
| 著者 | Kurata R, Adachi M, Yamakawa O, Yoshimoto M. |
| タイトル | Growth suppression of human cancer cells by polyphenolics from sweetpotato (Ipomoea batatas L.) leaves. |
| ジャーナル | J Agric Food Chem |
| Abstract |
Sweetpotato leaves (Ipomoea batatas L.) contain a high content of polyphenolics that consist of caffeic acid, chlorogenic acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, and 3,4,5-tri-O-caffeoylquinic acid. We investigated the suppression of the proliferation of selected human cancer cells by phenolic compounds isolated from sweetpotato leaf. The human cancer cells used in this research included a stomach cancer (Kato III), a colon cancer (DLD-1), and a promyelocytic leukemia cell (HL-60). Caffeic acid and di- and tricaffeoylquinic acids dose-dependently depressed cancer cell proliferation, and the difference in sensitivity between caffeoylquinic acid derivatives and each kind of cancer cell was observed. Specifically, 3,4,5-tri-O-caffeoylquinic acid effectively depressed the growth of three kinds of cancer cells, and caffeic acid had an exceptionally higher effect against HL-60 cells than other di- and tricaffeoylquinic acids. In attempting to clarify the mechanism of growth suppression with the addition of the apoptotic inhibitor N-ethylmaleimide, we observed that the nuclear granulation in 3,4,5-tri-O-caffeoylquinic acid-treated HL-60 cells suggested apoptosis induction. This effect was confirmed by DNA fragmentation, an increase of caspase-3 activity, and expression of c-Jun. Growth suppression of HL-60 cells by 3,4,5-tri-O-caffeoylquinic acid was determined to be the result of apoptotic death of the cells. These results indicate that 3,4,5-tri-O-caffeoylquinic acid may have potential for cancer prevention. |
| 巻・号 | 55(1) |
| ページ | 185-90 |
| 公開日 | 2007-1-10 |
| DOI | 10.1021/jf0620259 |
| PMID | 17199331 |
| MeSH | Apoptosis / drug effects Cell Division / drug effects* Cell Line, Tumor Colonic Neoplasms Flavonoids / pharmacology* HL-60 Cells Humans Ipomoea batatas / chemistry* Neoplasms / pathology* Phenols / pharmacology* Plant Leaves / chemistry* Polyphenols Reverse Transcriptase Polymerase Chain Reaction Stomach Neoplasms |
| IF | 4.192 |
| 引用数 | 120 |
| WOS 分野 | AGRICULTURE, MULTIDISCIPLINARY FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) |