RRC ID 42853
著者 Kimura Y, Sumiyoshi M, Suzuki T, Sakanaka M.
タイトル Antitumor and antimetastatic activity of a novel water-soluble low molecular weight beta-1, 3-D-glucan (branch beta-1,6) isolated from Aureobasidium pullulans 1A1 strain black yeast.
ジャーナル Anticancer Res
Abstract Though it has been reported that beta-glucans or protein-binding hetero-glucans isolated from mushrooms have antitumor activity, the antitumor and antimetastatic actions of purified, structurally defined polysaccharides (such as beta-glucans) have not been proven yet. A new low molecular weight (approximately 100 kDa) beta-glucan was isolated from Aureobasidium pullulans black yeast, and was found to have low viscosity and to be water-soluble. The industrial production of this P-glucan was achieved from the culture media of A. pullulans. The effects of water-soluble low-molecular-weight (LMW) beta-(1-->3) and 50-80% branched beta-(1-->6) glucan isolated from A. pullulans on tumor growth and metastasis to the liver were examined in mice intrasplenically with implanted colon 26 tumor cells. In addition, to clarify the antitumor and antimetastatic actions of LMW beta-(1,3-1,6) glucan, the effects on immune functions in the small intestine were also examined. The intraperitoneal (5 and 15 mg/kg) and oral (50 mg/kg) administration of LMW P-(1.3-1.6) glucan inhibited the tumor growth and liver metastasis in mice intrasplenically implanted with colon 26 cells. The numbers of natural killer (NK)- and interferon (IFN)-gamma-positive cells in the small intestine of colon 26-bearing mice were lower than those in normal mice. The intraperitoneally and orally administered LMW beta-(1,3-1,6) glucan prevented the reduction of the number of NK- and IFN-gamma-positive cells induced by the tumor growth after implantation of colon 26 cells. These findings suggest that the antitumor and antimetastatic actions of LMW beta-(1,3-1,6) glucan may involve the enhancement of intestinal immune functions through increases in NK- and IFN-gamma-positive cell numbers.
巻・号 26(6B)
ページ 4131-41
公開日 2006-1-1
PMID 17201124
MeSH Animals Antineoplastic Agents / pharmacology* Basidiomycota / chemistry* Carbohydrate Sequence Cell Line, Tumor Magnetic Resonance Spectroscopy Male Mice Mice, Inbred BALB C Molecular Sequence Data Molecular Weight Neoplasm Metastasis / prevention & control* Solubility Water beta-Glucans / chemistry beta-Glucans / pharmacology*
IF 1.994
引用数 44
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)