| RRC ID |
42872
|
| Author |
Mizutani T, Haga H, Kawabata K.
|
| Title |
Development of a device to stretch tissue-like materials and to measure their mechanical properties by scanning probe microscopy.
|
| Journal |
Acta Biomater
|
| Abstract |
We have developed a new stretch device to investigate the biomechanical responses to an external loading force on a tissue-like material consisting of cells and a collagen gel. Collagen gel, a typical matrix found abundantly in the connective tissue, was attached to an elastic chamber that was precoated with a thin layer of collagen. Madin-Darby canine kidney cells that were cultured on the collagen gel were stretched in a uniaxial direction via deformation of the elastic chamber. Changes in the morphology and stiffness of the tissue-like structure were measured before and after the stretch using wide-range scanning probe microscopy (WR-SPM). The change in cellular morphology was heterogeneous, and there was a twofold increase in the intercellular junction due to the stretch. In addition to the WR-SPM measurements, this device enables observation of the spatial distribution of cytoskeletal proteins such as vimentin and alpha-catenin using immunofluorescent microscopy. We concluded that the stretch device we have reported in this paper is useful for measuring the mechanical response of a tissue-like material over a range of cell sizes when exposed to an external loading force.
|
| Volume |
3(4)
|
| Pages |
485-93
|
| Published |
2007-7-1
|
| DOI |
10.1016/j.actbio.2006.11.007
|
| PII |
S1742-7061(06)00150-4
|
| PMID |
17251072
|
| MeSH |
Animals
Biomedical Engineering / instrumentation*
Biomedical Engineering / methods*
Cell Line
Coated Materials, Biocompatible / chemistry
Collagen / chemistry
Dogs
Epithelial Cells / physiology*
Epithelial Cells / ultrastructure*
Gels / chemistry
Microscopy, Scanning Probe / methods*
Stress, Mechanical
Vimentin / metabolism
alpha Catenin / metabolism
|
| IF |
7.242
|
| Times Cited |
6
|
|
WOS Category
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| Resource |
| Human and Animal Cells |
|
