RRC ID 42875
著者 Osaki T, Saito H, Yoshikawa T, Matsumoto S, Tatebe S, Tsujitani S, Ikeguchi M.
タイトル Decreased NKG2D expression on CD8+ T cell is involved in immune evasion in patients with gastric cancer.
ジャーナル Clin Cancer Res
Abstract PURPOSE:Some studies suggest that the immunoreceptor NKG2D expression on CD8(+) T cells is down-regulated and this reduction may be involved in immune evasion in cancer patients. The present study was designed to investigate NKG2D expression on CD8(+) T lymphocytes and its relationship to immune evasion in gastric cancer patients.
EXPERIMENTAL DESIGN:NKG2D expression on both circulating and tumor-infiltrating CD8(+) T cells was evaluated by multicolor flow cytometry. Soluble MHC class I chain-related gene A (MICA) in the sera was quantitated by ELISA. Transwell experiments were carried out to determine the effect of cancer cells on NKG2D expression.
RESULTS:NKG2D expression on circulating CD8(+) T cells was down-regulated and significantly correlated with IFN-gamma production in gastric cancer patients (r = 0.68; P = 0.007). NKG2D expression was closely related to undifferentiated cancer (P = 0.021) as was the depth of invasion (P = 0.012). There was no difference in soluble MICA between gastric cancer patients and normal controls. NKG2D expression on CD8(+) T cells was remarkably reduced in the tissue of gastric cancer compared with peripheral blood (P = 0.046). Complete removal of tumor by surgery restored NKG2D expression on CD8(+) T cells (P = 0.0049). Transwell experiments showed that this down-regulation was induced by direct contact between cancer cells and CD8(+) T cells and that soluble factors did not affect the NKG2D expression. This phenomenon was blocked by the addition of anti-MICA antibodies.
CONCLUSIONS:Decreased NKG2D expression may be one of the key mechanisms responsible for immune evasion by tumors in gastric cancer.
巻・号 13(2 Pt 1)
ページ 382-7
公開日 2007-1-15
DOI 10.1158/1078-0432.CCR-06-1454
PII 13/2/382
PMID 17255258
MeSH Adult Aged CD8-Positive T-Lymphocytes / metabolism* Cell Differentiation Female Gene Expression Regulation, Neoplastic* Genes, MHC Class I Humans Interferon-gamma / biosynthesis Male Middle Aged NK Cell Lectin-Like Receptor Subfamily K Receptors, Immunologic / biosynthesis* Receptors, Natural Killer Cell Stomach Neoplasms / immunology* Stomach Neoplasms / metabolism Stomach Neoplasms / pathology* Time Factors
IF 10.107
引用数 41
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞