Reference - Detail
| RRC ID | 42891 |
|---|---|
| Author | Enosawa S, Miyashita T, Saito T, Omasa T, Matsumura T. |
| Title | The significant improvement of survival times and pathological parameters by bioartificial liver with recombinant HepG2 in porcine liver failure model. |
| Journal | Cell Transplant |
| Abstract |
We developed a bioartificial liver (BAL) containing human hepatoblastoma cell line, HepG2, with the addition of ammonia removal activity by transfecting a glutamine synthetase (GS) gene and estimated the efficacy using pigs with ischemic liver failure. GS-HepG2 cells showed 15% ammonia removal activity of porcine hepatocytes, while unmodified HepG2 had no such activity. The established GS-HepG2 cells were grown in a circulatory flow bioreactor to 3.5-4.1 x 10(9) cells. Survival time of the animals treated with GS-HepG2 BAL was significantly prolonged compared to the cell-free control (14.52 +/- 5.24 h vs. 8.53 +/- 2.52 h) and the group treated with the BAL consisting of unmodified wild-type HepG2 (9.58 +/- 4.52 h). Comparison showed the cell-containing BAL groups to have significantly fewer incidences of increased brain pressure. Thus, the GS-HepG2 BAL treatment resulted in a significant improvement of survival time and pathological parameters in pigs with ischemic liver failure. |
| Volume | 15(10) |
| Pages | 873-80 |
| Published | 2006-1-1 |
| DOI | 10.3727/000000006783981350 |
| PMID | 17299991 |
| MeSH | Animals Bioreactors Cell Culture Techniques Cell Line, Tumor Cell Transplantation Cricetinae Disease Models, Animal Female Glutamate-Ammonia Ligase / genetics Glutamate-Ammonia Ligase / metabolism* Humans Liver Failure / metabolism Liver Failure / mortality Liver Failure / therapy* Liver, Artificial* Male Models, Biological Survival Rate Swine Transfection |
| IF | 3.341 |
| Times Cited | 23 |
| WOS Category | TRANSPLANTATION MEDICINE, RESEARCH & EXPERIMENTAL CELL & TISSUE ENGINEERING |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Hep G2(RCB0459) |