| RRC ID |
42891
|
| Author |
Enosawa S, Miyashita T, Saito T, Omasa T, Matsumura T.
|
| Title |
The significant improvement of survival times and pathological parameters by bioartificial liver with recombinant HepG2 in porcine liver failure model.
|
| Journal |
Cell Transplant
|
| Abstract |
We developed a bioartificial liver (BAL) containing human hepatoblastoma cell line, HepG2, with the addition of ammonia removal activity by transfecting a glutamine synthetase (GS) gene and estimated the efficacy using pigs with ischemic liver failure. GS-HepG2 cells showed 15% ammonia removal activity of porcine hepatocytes, while unmodified HepG2 had no such activity. The established GS-HepG2 cells were grown in a circulatory flow bioreactor to 3.5-4.1 x 10(9) cells. Survival time of the animals treated with GS-HepG2 BAL was significantly prolonged compared to the cell-free control (14.52 +/- 5.24 h vs. 8.53 +/- 2.52 h) and the group treated with the BAL consisting of unmodified wild-type HepG2 (9.58 +/- 4.52 h). Comparison showed the cell-containing BAL groups to have significantly fewer incidences of increased brain pressure. Thus, the GS-HepG2 BAL treatment resulted in a significant improvement of survival time and pathological parameters in pigs with ischemic liver failure.
|
| Volume |
15(10)
|
| Pages |
873-80
|
| Published |
2006-1-1
|
| DOI |
10.3727/000000006783981350
|
| PMID |
17299991
|
| MeSH |
Animals
Bioreactors
Cell Culture Techniques
Cell Line, Tumor
Cell Transplantation
Cricetinae
Disease Models, Animal
Female
Glutamate-Ammonia Ligase / genetics
Glutamate-Ammonia Ligase / metabolism*
Humans
Liver Failure / metabolism
Liver Failure / mortality
Liver Failure / therapy*
Liver, Artificial*
Male
Models, Biological
Survival Rate
Swine
Transfection
|
| IF |
3.341
|
| Times Cited |
23
|
|
WOS Category
|
TRANSPLANTATION
MEDICINE, RESEARCH & EXPERIMENTAL
CELL & TISSUE ENGINEERING
|
| Resource |
| Human and Animal Cells |
Hep G2(RCB0459) |
