| RRC ID |
42907
|
| Author |
Notoya M, Arai R, Katafuchi T, Minamino N, Hagiwara H.
|
| Title |
A novel member of the calcitonin gene-related peptide family, calcitonin receptor-stimulating peptide, inhibits the formation and activity of osteoclasts.
|
| Journal |
Eur J Pharmacol
|
| Abstract |
We isolated a novel peptide, calcitonin receptor-stimulating peptide-1 (CRSP-1), from porcine brain and found that the administration of this peptide into rats induced a transient decrease in plasma calcium concentration. Therefore, we investigated the effects of CRSP-1 on osteoclastogenesis. Osteoclast-like cells were formed from spleen cells or bone marrow cells by a combination of the receptor activator of nuclear factor-kappaB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). CRSP-1 dose-dependently inhibited the formation of multinucleated osteoclast-like cells, and a calcitonin receptor inhibitor antagonized in part the inhibition of osteoclast formation by CRSP-1. Furthermore, CRSP-1 destroyed the actin ring that is a typical index of osteoclast resorption activity; it contributed to this action via the signaling pathway of protein kinase A. Our findings indicate that CRSP-1 inhibits osteoclastogenesis by inhibiting the formation and activity of multinucleated osteoclasts. The inhibitory effects of CRSP-1 on osteoclast metabolism were similar in degree to those of porcine calcitonin. CRSP-1 might provide a clue to the development of tools useful in the prevention and treatment of osteoporosis.
|
| Volume |
560(2-3)
|
| Pages |
234-9
|
| Published |
2007-4-10
|
| DOI |
10.1016/j.ejphar.2007.01.034
|
| PII |
S0014-2999(07)00080-5
|
| PMID |
17328890
|
| MeSH |
Actins / drug effects
Animals
Calcitonin Gene-Related Peptide / pharmacology*
Cyclic AMP / biosynthesis
Male
Mice
Osteoclasts / drug effects*
Osteoclasts / physiology
Osteoporosis / drug therapy
Receptors, Calcitonin / biosynthesis*
|
| IF |
3.263
|
| Times Cited |
20
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
|
| Resource |
| Human and Animal Cells |
ST2(RCB0224) |
