| RRC ID |
42913
|
| Author |
Morikawa A, Hamase K, Ohgusu T, Etoh S, Tanaka H, Koshiishi I, Shoyama Y, Zaitsu K.
|
| Title |
Immunohistochemical localization of D-alanine to beta-cells in rat pancreas.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
A mouse monoclonal antibody against D-alanine (D-Ala) has been raised and the immunohistochemical localization of this D-amino acids in the rat pancreas is visualized. The obtained anti-D-Ala monoclonal antibody has no significant cross-reactivity to all proteinogenic L-amino acids and their D-enantiomers. Using this antibody, immunohistochemical staining was performed on the pancreas, and specific staining for d-Ala has been observed only in the Langerhans islets. To identify the types of D-Ala-immunopositive cells, double staining was carried out with antibodies against D-Ala and pancreatic hormones. Similar immunostaining patterns have been observed for D-Ala and insulin, while D-Ala is hardly co-localized with other hormones (glucagon, somatostatin, and pancreatic polypeptide). These results indicate for the first time that D-Ala is localized to insulin producing beta-cells in mammalian pancreas, suggesting that this D-amino acid would be involved in the regulation of the blood glucose level.
|
| Volume |
355(4)
|
| Pages |
872-6
|
| Published |
2007-4-20
|
| DOI |
10.1016/j.bbrc.2007.02.056
|
| PII |
S0006-291X(07)00277-X
|
| PMID |
17336268
|
| MeSH |
Alanine / immunology
Alanine / metabolism*
Animals
Antibodies, Monoclonal / immunology
Enzyme-Linked Immunosorbent Assay
Female
Immunoglobulin G / immunology
Immunohistochemistry
Insulin-Secreting Cells / metabolism*
Male
Mice
Rats
|
| IF |
2.985
|
| Times Cited |
40
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
SP2/0-Ag14(RCB0209) |
