| RRC ID |
42930
|
| 著者 |
Odashima M, Otaka M, Matsuhashi T, Jin M, Horikawa Y, Ohba R, Itoh H, Watanabe S.
|
| タイトル |
Mechanical strain stress suppresses expression of HSP70 and wound restoration in gastric mucosal cells.
|
| ジャーナル |
Dig Dis Sci
|
| Abstract |
The major heat shock protein, HSP70, is known to be involved in cytoprotection against environmental stresses mediated by their function as a "molecular chaperone." However, the influence of HSP70 on gastric mucosal healing under physical stimulation or stress is not completely understood. Rat gastric mucosal cells (RGM-1) were stably transfected with pBK-CMV containing the human HSP70 gene (7018-RGM-1) or pBK-CMV alone (pBK-CMV-12). Artificial wounds were created. Mechanical stretch was applied to 7018-RGM-1 cells or pBK-CMV-12 cells. The effect of mechanical stretch on HSP70 expression was assessed by Western blot analysis. Expression of HSP70 was decreased by mechanical stretch in pBK-CMV-12 cells. However, expression of HSP70 was not decreased by mechanical stretch in 7018-RGM-1 cells. Furthermore, the wound restoration of pBK-CMV-12 cells was suppressed under mechanical stretch condition. On the other hand, the wound restoration of 7018-RGM-1 cells was not affected by mechanical stretch. These results suggest that HSP70 plays an important role in gastric wound healing under physical stress.
|
| 巻・号 |
52(11)
|
| ページ |
3087-91
|
| 公開日 |
2007-11-1
|
| DOI |
10.1007/s10620-007-9795-3
|
| PMID |
17393320
|
| MeSH |
Animals
Biomarkers / metabolism
Blotting, Western
Cell Line
Cytomegalovirus / genetics
DNA / genetics
Electrophoresis, Polyacrylamide Gel
Enterocytes / metabolism
Enterocytes / pathology
Gastric Mucosa / injuries
Gastric Mucosa / metabolism
Gastric Mucosa / pathology*
HSP70 Heat-Shock Proteins / biosynthesis*
HSP70 Heat-Shock Proteins / genetics
Humans
Rats
Stress, Mechanical
Transfection
Wound Healing / physiology*
|
| IF |
2.751
|
| 引用数 |
9
|
|
WOS 分野
|
GASTROENTEROLOGY & HEPATOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RGM1(RCB0876) |
