RRC ID 42945
Author Zhang J, Takeda M.
Title Molecular characterization of MbADGF, a novel member of the adenosine deaminase-related growth factor in the cabbage armyworm, Mamestra brassicae: the functional roles in the midgut cell proliferation.
Journal Insect Mol Biol
Abstract To clarify the functional mechanism of the adenosine deaminase-related growth factor (ADGF) particularly in the regulation of insect development, the cDNA encoding a homologue of ADGF proteins was cloned from the cabbage armyworm, Mamestra brassicae, named MbADGF. The purified MbADGF recombinant protein stimulated cell proliferation in a dose-dependent manner of SES-MaBr-4 and NIAS-MaBr-93 cell lines that were derived from fat bodies and haemocytes of M. brassicae. The adenosine deaminase activity of MbADGF was detected using adenosine and 2'-deoxyadenosine as substrates. Northern analysis demonstrated that during the larval development the level of MbADGF in the midgut increased. In situ hybridization showed that MbADGF mRNA was expressed in midgut goblet cells and in the apical cytoplasm of columnar cells, which suggests that MbADGF protein may execute its adenosine deaminase activity at the apical cytoplasm of columnar cells to convert adenosine into inosine.
Volume 16(3)
Pages 351-60
Published 2007-6
DOI 10.1111/j.1365-2583.2007.00732.x
PII IMB732
PMID 17439545
MeSH Adenosine / metabolism Amino Acid Sequence Animals Base Sequence Blotting, Northern Cell Line Cell Proliferation / drug effects* Cluster Analysis DNA Primers / genetics DNA, Complementary / genetics Deoxyadenosines / metabolism Digestive System / cytology* Digestive System / metabolism Dose-Response Relationship, Drug In Situ Hybridization Insect Proteins / genetics* Intercellular Signaling Peptides and Proteins / genetics* Larva / metabolism Molecular Sequence Data Moths / genetics* Moths / metabolism Phylogeny Recombinant Proteins / genetics Recombinant Proteins / pharmacology* Sequence Alignment Sequence Analysis, DNA
IF 2.437
Times Cited 5
WOS Category ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Human and Animal Cells