RRC ID |
42945
|
著者 |
Zhang J, Takeda M.
|
タイトル |
Molecular characterization of MbADGF, a novel member of the adenosine deaminase-related growth factor in the cabbage armyworm, Mamestra brassicae: the functional roles in the midgut cell proliferation.
|
ジャーナル |
Insect Mol Biol
|
Abstract |
To clarify the functional mechanism of the adenosine deaminase-related growth factor (ADGF) particularly in the regulation of insect development, the cDNA encoding a homologue of ADGF proteins was cloned from the cabbage armyworm, Mamestra brassicae, named MbADGF. The purified MbADGF recombinant protein stimulated cell proliferation in a dose-dependent manner of SES-MaBr-4 and NIAS-MaBr-93 cell lines that were derived from fat bodies and haemocytes of M. brassicae. The adenosine deaminase activity of MbADGF was detected using adenosine and 2'-deoxyadenosine as substrates. Northern analysis demonstrated that during the larval development the level of MbADGF in the midgut increased. In situ hybridization showed that MbADGF mRNA was expressed in midgut goblet cells and in the apical cytoplasm of columnar cells, which suggests that MbADGF protein may execute its adenosine deaminase activity at the apical cytoplasm of columnar cells to convert adenosine into inosine.
|
巻・号 |
16(3)
|
ページ |
351-60
|
公開日 |
2007-6-1
|
DOI |
10.1111/j.1365-2583.2007.00732.x
|
PII |
IMB732
|
PMID |
17439545
|
MeSH |
Adenosine / metabolism
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Cell Line
Cell Proliferation / drug effects*
Cluster Analysis
DNA Primers / genetics
DNA, Complementary / genetics
Deoxyadenosines / metabolism
Digestive System / cytology*
Digestive System / metabolism
Dose-Response Relationship, Drug
In Situ Hybridization
Insect Proteins / genetics*
Intercellular Signaling Peptides and Proteins / genetics*
Larva / metabolism
Molecular Sequence Data
Moths / genetics*
Moths / metabolism
Phylogeny
Recombinant Proteins / genetics
Recombinant Proteins / pharmacology*
Sequence Alignment
Sequence Analysis, DNA
|
IF |
2.533
|
引用数 |
5
|
WOS 分野
|
ENTOMOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
|