論文 - 詳細
| RRC ID | 42953 |
|---|---|
| 著者 | Sawada N, Taketani Y, Amizuka N, Ichikawa M, Ogawa C, Nomoto K, Nashiki K, Sato T, Arai H, Isshiki M, Segawa H, Yamamoto H, Miyamoto K, Takeda E. |
| タイトル | Caveolin-1 in extracellular matrix vesicles secreted from osteoblasts. |
| ジャーナル | Bone |
| Abstract |
Caveolin-1 is an essential and signature protein of caveolae, which are small invaginations of the plasma membrane enriched in cholesterol and sphingolipids. Although high levels of expression of caveolin-1 have been demonstrated in osteoblasts as well as endothelial cells, fibroblasts, and muscular cells, the role of caveolin-1 in osteoblasts has not been clarified. Here, we show that caveolin-1 is secreted from osteoblasts in the form of matrix vesicles; extracellular vesicles released from the plasma membrane of osteoblasts. In this study, caveolae and matrix vesicles were similarly enriched in cholesterol and sphingomyelin in fractions isolated from mineralizing MC3T3-E1 cells. Interestingly, in the MC3T3-E1 cells caveolin-1 was enriched in the matrix vesicle fraction as well as the caveolar membrane fraction, and the amount of caveolin-1 in the matrix vesicle fraction increased as differentiation progressed. Localization of caveolin-1 in matrix vesicles was also confirmed in murine tibia. Furthermore, overexpression of caveolin-1 enhanced matrix calcification in MC3T3-E1 cells, whereas knockdown of caveolin-1 diminished it. These results suggest that secreted caveolin-1 as a component of matrix vesicles may play an important role in osteoblast calcification. |
| 巻・号 | 41(1) |
| ページ | 52-8 |
| 公開日 | 2007-7-1 |
| DOI | 10.1016/j.bone.2007.02.030 |
| PII | S8756-3282(07)00101-9 |
| PMID | 17448744 |
| MeSH | 3T3 Cells Alkaline Phosphatase / metabolism Animals Base Sequence Calcification, Physiologic Caveolin 1 / antagonists & inhibitors Caveolin 1 / genetics Caveolin 1 / metabolism* Cell Differentiation Cholesterol / metabolism DNA Primers / genetics Extracellular Matrix / metabolism Gene Expression Mice Microscopy, Immunoelectron Osteoblasts / metabolism* Osteoblasts / ultrastructure Phosphates / metabolism RNA Interference Secretory Vesicles / metabolism Sphingomyelins / metabolism Tibia / metabolism Tibia / ultrastructure |
| IF | 4.147 |
| 引用数 | 20 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |