論文 - 詳細
| RRC ID | 42954 |
|---|---|
| 著者 | Soh H, Wasa M, Fukuzawa M. |
| タイトル | Hypoxia upregulates amino acid transport in a human neuroblastoma cell line. |
| ジャーナル | J Pediatr Surg |
| Abstract |
BACKGROUND/PURPOSE:Some tumor cells survive and even grow in hypoxic conditions. We examined the effect of hypoxia on amino acid transport in a human neuroblastoma cell line SK-N-SH. METHODS:Cells were incubated under hypoxic conditions (1% O2-5% CO2-94% N2). After 0, 8, 16, and 24 hours, the transport of 3H-glutamine, 3H-glutamate, and 3H-leucine was assayed. 3H-Thymidine and 3H-leucine incorporation was measured for the determination of DNA and protein synthesis, respectively. RESULTS:Hypoxia increased Na+-dependent glutamine and Na+-independent leucine transport significantly at 16 and 24 hours compared with control (P < .01) by a mechanism that increased Vmax without affecting Km. These increases were completely blocked by actinomycin D and cycloheximide. There was no significant difference in Na+-dependent glutamate transport between control and hypoxic groups. DNA and protein synthesis significantly decreased in the hypoxic condition compared with control (P < .01). CONCLUSIONS:This study demonstrated that hypoxia upregulates amino acid transport in a human neuroblastoma cell line. This mechanism may allow cells to survive and even grow under hypoxic conditions. |
| 巻・号 | 42(4) |
| ページ | 608-12 |
| 公開日 | 2007-4-1 |
| DOI | 10.1016/j.jpedsurg.2006.12.010 |
| PII | S0022-3468(06)00940-7 |
| PMID | 17448754 |
| MeSH | Amino Acids / metabolism* Biological Transport Cell Hypoxia Cycloheximide / pharmacology Dactinomycin / pharmacology Glutamic Acid / metabolism Glutamine / metabolism Humans Hypoxia / metabolism* Leucine / metabolism Neuroblastoma / metabolism* Protein Synthesis Inhibitors / pharmacology Tumor Cells, Cultured Up-Regulation* |
| IF | 1.919 |
| 引用数 | 14 |
| WOS 分野 | PEDIATRICS SURGERY |
| リソース情報 | |
| ヒト・動物細胞 | SK-N-SH(RCB0426) |