| RRC ID |
42955
|
| Author |
Ohba S, Nakajima K, Komiyama Y, Kugimiya F, Igawa K, Itaka K, Moro T, Nakamura K, Kawaguchi H, Takato T, Chung UI.
|
| Title |
A novel osteogenic helioxanthin-derivative acts in a BMP-dependent manner.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
To effectively treat serious bone defects using bone regenerative medicine, there is a need for the development of a small chemical compound that potently induces bone formation. We now report a novel osteogenic helioxanthin-derivative, TH. TH induced osteogenic differentiation in MC3T3-E1 cells, mouse primary osteoblasts, and mouse embryonic stem cells. The combination of TH and bone morphogenetic protein (BMP) 2 induced the mRNA expression of osteoblast marker genes and calcification in primary fibroblasts. The TH induced the mRNA of the inhibitor of DNA-binding 1 (Id-1), and its osteogenic effect was inhibited by Smad6 or Noggin. Furthermore, TH induced the mRNA expression of Bmp4 and Bmp6. These data suggest that TH exerts its potent osteogenic effect in a BMP-dependent manner by enhancing the effects of the existing BMPs and/or increasing the expression of Bmp4 and Bmp6. TH may help establish a more efficient bone regeneration system.
|
| Volume |
357(4)
|
| Pages |
854-60
|
| Published |
2007-6-15
|
| DOI |
10.1016/j.bbrc.2007.03.173
|
| PII |
S0006-291X(07)00669-9
|
| PMID |
17451649
|
| MeSH |
3T3 Cells
Animals
Bone Morphogenetic Protein 2
Bone Morphogenetic Proteins / administration & dosage*
Cell Differentiation / drug effects
Dose-Response Relationship, Drug
Drug Combinations
Lignans
Mice
Osteoblasts / cytology*
Osteoblasts / drug effects*
Osteogenesis / drug effects*
Osteogenesis / physiology
Transforming Growth Factor beta / administration & dosage*
Xanthines / administration & dosage*
|
| IF |
2.985
|
| Times Cited |
32
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126)
NIH3T3 |
