| RRC ID |
42959
|
| Author |
Ishikawa M, Kitayama J, Yamauchi T, Kadowaki T, Maki T, Miyato H, Yamashita H, Nagawa H.
|
| Title |
Adiponectin inhibits the growth and peritoneal metastasis of gastric cancer through its specific membrane receptors AdipoR1 and AdipoR2.
|
| Journal |
Cancer Sci
|
| Abstract |
Adiponectin, a circulating peptide hormone produced in adipose tissue, has been shown to be reduced in the plasma of patients with cancer, suggesting that this adipokine may be mechanically involved in the pathogenesis of adiposity-related carcinogenesis. In this study, we examined the expression of adiponectin receptors (AdipoR1 and AdipoR2) and assessed the function of adiponectin in gastric cancer. All of the six gastric cancer cell lines significantly expressed mRNA and protein of both receptors with variable levels. Addition of 30 microg/mL adiponectin potently induced apoptosis and inhibited the proliferation of AZ521 and HCG27. Down-regulation of either AdipoR1 or AdipoR2 by specific siRNA significantly suppressed the growth inhibitory effects of adiponectin in both cell lines. Moreover, a local injection of adiponectin markedly inhibited the growth of AZ521 inoculated subcutaneously in nude mice. Similarly, the continuous intraperitoneal infusion of adiponectin effectively suppressed the development of peritoneal metastasis of AZ521. Adiponectin negatively regulates the progression of gastric cancer cells possibly through both AdipoR1 and AdipoR2. Although adiponectin was already reported to have antiangiogenic effects, our results suggest that the antitumor effect of adiponectin was, at least partially, dependent on the direct effects on tumor cells.
|
| Volume |
98(7)
|
| Pages |
1120-7
|
| Published |
2007-7-1
|
| DOI |
10.1111/j.1349-7006.2007.00486.x
|
| PII |
CAS486
|
| PMID |
17459059
|
| MeSH |
Adiponectin / therapeutic use*
Animals
Apoptosis / drug effects
Cell Division / drug effects
Cell Line, Tumor
Disease Progression
Female
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
RNA, Small Interfering / genetics
Receptors, Adiponectin
Receptors, Cell Surface / genetics
Receptors, Cell Surface / physiology*
Recombinant Proteins / therapeutic use
Reverse Transcriptase Polymerase Chain Reaction
Stomach Neoplasms / blood supply
Stomach Neoplasms / pathology*
|
| IF |
4.966
|
| Times Cited |
100
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
MKN28(RCB1000)
MKN45(RCB1001)
MKN74(RCB1002)
HGC-27(RCB500) |
