Reference - Detail
| RRC ID | 42968 |
|---|---|
| Author | Yamada P, Isoda H, Han JK, Talorete TP, Abe Y. |
| Title | Inhibitory effect of fulvic acid extracted from Canadian sphagnum peat on chemical mediator release by RBL-2H3 and KU812 cells. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Fulvic acid (FA) was extracted and purified from Canadian Sphagnum peat (CP-FA) and characterized by using an element analysis meter, Fourier transform infrared (FT-IR) spectroscopy, electron spin resonance (ESR) spectroscopy, and (13)C-nuclear magnetic resonance ((13)C-NMR) spectroscopy. To investigate the antiallergic effect of CP-FA, we incubated rat basophilic leukemia (RBL-2H3) cells with 0.001-10.0 microg/ml of CP-FA and determined the beta-hexosaminidase release inhibition at different response stages. The intracellular calcium [Ca(2+)](i) level was also determined by using Fluo 3-AM, a calcium-specific fluorescent probe, and the cytotoxicity of CP-FA was determined by the 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. The results revealed that RBL-2H3 cells incubated for 48 h with 0.001-10.0 microg/ml of CP-FA did not show any decreased viability. CP-FA inhibited the beta-hexosaminidase release by IgE-sensitized, antigen-stimulated RBL-2H3 cells at the antigen-antibody binding stage and the antibody-receptor binding stage. CP-FA also inhibited histamine release from A23187 plus PMA- or compound 48/80-stimulated KU812 cells. Furthermore, there was a decrease in the intracellular [Ca(2+)](i) level in IgE-sensitized cells incubated with CP-FA and stimulated with antigen. Our results show that CP-FA may be useful for the treatment or prevention of allergic diseases. |
| Volume | 71(5) |
| Pages | 1294-305 |
| Published | 2007-5-1 |
| DOI | 10.1271/bbb.60702 |
| PII | JST.JSTAGE/bbb/60702 |
| PMID | 17485833 |
| MeSH | Animals Anti-Allergic Agents / pharmacology* Antigen-Antibody Complex / immunology Antigens / immunology Basophils / metabolism* Benzopyrans / pharmacology* Calcimycin / pharmacology Calcium / analysis Cell Line, Tumor Dose-Response Relationship, Drug Fluorescent Dyes Formazans / metabolism Histamine Release / drug effects* Humans Immunoglobulin E / pharmacology Ionophores / pharmacology Leukemia, Basophilic, Acute Leukemia, Experimental Leukemia, Myelogenous, Chronic, BCR-ABL Positive Nuclear Magnetic Resonance, Biomolecular Plant Extracts / pharmacology* Rats Spectroscopy, Fourier Transform Infrared Sphagnopsida / chemistry* Tetradecanoylphorbol Acetate / pharmacology Tetrazolium Salts / metabolism beta-N-Acetylhexosaminidases / antagonists & inhibitors p-Methoxy-N-methylphenethylamine / pharmacology |
| IF | 1.516 |
| Times Cited | 38 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RBL 2H3(RCB2782) |