| RRC ID |
42987
|
| Author |
Hirata N, Naruto S, Ohguchi K, Akao Y, Nozawa Y, Iinuma M, Matsuda H.
|
| Title |
Mechanism of the melanogenesis stimulation activity of (-)-cubebin in murine B16 melanoma cells.
|
| Journal |
Bioorg Med Chem
|
| Abstract |
(-)-Cubebin showed a melanogenesis stimulation activity in a concentration-dependent manner in murine B16 melanoma cells without any significant effects on cell proliferation. Tyrosinase activity was increased at 24-72 h after addition of cubebin to B16 cells, and then intracellular melanin amount was increased at 48-96 h after the treatment. The expression levels of tyrosinase were time-dependently enhanced after the treatment with cubebin. At the same time, the expression levels of tyrosinase mRNA were also increased after addition of cubebin. Furthermore Western blot analysis revealed that cubebin elevated the level of phosphorylation of p38 mitogen-activated protein kinase (MAPK). SB203580, a selective inhibitor of p38 MAPK, completely blocked cubebin-induced expression of tyrosinase mRNA in B16 cells. These results suggested that cubebin increased melanogenesis in B16 cells through the enhancement of tyrosinase expression mediated by activation of p38 MAPK.
|
| Volume |
15(14)
|
| Pages |
4897-902
|
| Published |
2007-7-15
|
| DOI |
10.1016/j.bmc.2007.04.046
|
| PII |
S0968-0896(07)00373-2
|
| PMID |
17521910
|
| MeSH |
Animals
Cell Line, Tumor
Cell Proliferation / drug effects
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Enzymologic / genetics
Imidazoles / pharmacology
Lignans / pharmacology*
Melanins / biosynthesis*
Melanoma / metabolism*
Melanoma / pathology
Mice
Monophenol Monooxygenase / genetics
Monophenol Monooxygenase / metabolism
Phosphorylation / drug effects
Pyridines / pharmacology
RNA, Messenger / genetics
p38 Mitogen-Activated Protein Kinases / metabolism
|
| IF |
3.073
|
| Times Cited |
56
|
|
WOS Category
|
CHEMISTRY, MEDICINAL
CHEMISTRY, ORGANIC
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
|
