論文 - 詳細
| RRC ID | 43001 |
|---|---|
| 著者 | Nagaoka M, Ise H, Harada I, Koshimizu U, Maruyama A, Akaike T. |
| タイトル | Embryonic undifferentiated cells show scattering activity on a surface coated with immobilized E-cadherin. |
| ジャーナル | J Cell Biochem |
| Abstract |
Rearrangement of cell-cell adhesion is a critical event in embryonic development and tissue formation. We investigated the regulatory function of E-cadherin, a key adhesion protein, in the developmental process by using E-cadherin/IgG Fc fusion protein as an adhesion matrix in cell culture. F9 embryonal carcinoma cells usually form colonies when cultured on gelatin or fibronectin matrices. However, F9 cells cultured on the E-cadherin/IgG Fc fusion protein matrix formed a scattered distribution, with a different cytoskeletal organization and E-cadherin-rich protrusions that were regulated by Rac1 activity. The same scattering activity was observed in P19 embryonal carcinoma cells. In contrast, three types of differentiated cells, NMuMG mammary gland cells, MDCK kidney epithelial cells, and mouse primary isolated hepatocytes, did not show the scattering activity observed in F9 and P19 cells. These results suggest that migratory behavior on an E-cadherin-immobilized surface is only observed in embryonic cells, and that the regulatory mechanisms underlying E-cadherin-mediated cell adhesion vary with the state of differentiation. |
| 巻・号 | 103(1) |
| ページ | 296-310 |
| 公開日 | 2008-1-1 |
| DOI | 10.1002/jcb.21406 |
| PMID | 17559080 |
| MeSH | Actins / metabolism Cadherins / metabolism* Cell Adhesion Cell Differentiation Cell Line Cell Movement* Embryo, Mammalian / cytology* Humans Surface Properties rac1 GTP-Binding Protein / metabolism |
| IF | 4.237 |
| 引用数 | 22 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MDCK(RCB0995) |