| RRC ID |
43021
|
| 著者 |
Zhu L, Ye X, Tang G, Zhao N, Gong Y, Zhao Y, Zhao J, Zhang X.
|
| タイトル |
Biomimetic coating of compound titania and hydroxyapatite on titanium.
|
| ジャーナル |
J Biomed Mater Res A
|
| Abstract |
The modification on the titanium implant surface is an effective method to improve the biocompatibility of titanium. This article describes efforts to improve implant biocompatibility by applying titania and hydroxyapatite to form a three-layer coating on the titanium surface. This three-layer coating is made up of HA as the top layer (formed by hydrothermal treatment), porous TiO2 as the middle layer (formed by micro-arc oxidation) and a dense TiO2 film as the inner layer (formed by preanodic oxidation). The physicochemical characteristics, cell behavior and in vivo studies were assessed. The physicochemical characteristics were investigated using scanning electron micoscopy observation, fibronectin and laminin adsorption, corrosion test and X-ray diffraction analysis. Cell behavior included morphology observation with scanning electron microscopy (SEM), number count with methylthiazol tetrazolium (MTT) assay and Alkaline phosphatase (ALP, a representative enzyme of osteoblastic differentiation) activity of osteoblast-like MC3T3-E1 cells. In study in vivo the specimens were embedded in skull wound for repair. By the analysis of experiments, the titanium coated with this three-layer coating has been proved to have excellent corrosion resistance and good biocompatibility, which can promote cell proliferation and bone formation. So this modified titanium is an improved alternative to untreated titanium for bone repair applications.
|
| 巻・号 |
83(4)
|
| ページ |
1165-1175
|
| 公開日 |
2007-12-15
|
| DOI |
10.1002/jbm.a.31401
|
| PMID |
17595022
|
| MeSH |
3T3 Cells
Adsorption
Alkaline Phosphatase / metabolism
Animals
Biocompatible Materials
Durapatite / chemistry*
Mice
Microscopy, Electron, Scanning
Molecular Mimicry*
Proteins / chemistry
Rabbits
Titanium / chemistry*
X-Ray Diffraction
|
| IF |
3.525
|
| 引用数 |
40
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
