| RRC ID |
43022
|
| Author |
Islam S, Hassan F, Tumurkhuu G, Dagvadorj J, Koide N, Naiki Y, Mori I, Yoshida T, Yokochi T.
|
| Title |
Bacterial lipopolysaccharide induces osteoclast formation in RAW 264.7 macrophage cells.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Lipopolysaccharide (LPS) is a potent bone resorbing factor. The effect of LPS on osteoclast formation was examined by using murine RAW 264.7 macrophage cells. LPS-induced the formation of multinucleated giant cells (MGC) in RAW 264.7 cells 3 days after the exposure. MGCs were positive for tartrate-resistant acid phosphatase (TRAP) activity. Further, MGC formed resorption pits on calcium-phosphate thin film that is a substrate for osteoclasts. Therefore, LPS was suggested to induce osteoclast formation in RAW 264.7 cells. LPS-induced osteoclast formation was abolished by anti-tumor necrosis factor (TNF)-alpha antibody, but not antibodies to macrophage-colony stimulating factor (M-CSF) and receptor activator of nuclear factor (NF)-kappaB ligand (RANKL). TNF-alpha might play a critical role in LPS-induced osteoclast formation in RAW 264.7 cells. Inhibitors of NF-kappaB and stress activated protein kinase (SAPK/JNK) prevented the LPS-induced osteoclast formation. The detailed mechanism of LPS-induced osteoclast formation is discussed.
|
| Volume |
360(2)
|
| Pages |
346-51
|
| Published |
2007-8-24
|
| DOI |
10.1016/j.bbrc.2007.06.023
|
| PII |
S0006-291X(07)01257-0
|
| PMID |
17597583
|
| MeSH |
Animals
Bone Resorption / metabolism*
Bone Resorption / pathology
Cell Differentiation / drug effects
Cell Differentiation / physiology
Cell Line
Escherichia coli / metabolism*
Lipopolysaccharides / administration & dosage
Lipopolysaccharides / metabolism*
Macrophages / drug effects
Macrophages / metabolism*
Macrophages / pathology
Mice
Osteoclasts / drug effects*
Osteoclasts / pathology*
|
| IF |
2.985
|
| Times Cited |
82
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
