| RRC ID |
43040
|
| Author |
Kawate H, Wu Y, Ohnaka K, Takayanagi R.
|
| Title |
Mutual transactivational repression of Runx2 and the androgen receptor by an impairment of their normal compartmentalization.
|
| Journal |
J Steroid Biochem Mol Biol
|
| Abstract |
Steroid hormones play important roles not only in the reproductive system but also in bone metabolism. We examined the functional relationship between steroid hormone receptors and the Runx2 transcription factor that is essential for osteoblast differentiation and proliferation. A functional reporter assay using promoters carrying steroid hormone-responsive elements revealed that Runx2 suppressed ligand-dependent transcriptional activation mediated by receptors. To examine intracellular localization of these proteins, a three-dimensional imaging study was performed by laser scanning confocal microscopy of green fluorescent protein (GFP)-fused proteins. As previously reported, ligand-bound human androgen receptor (AR) was translocated from the cytoplasm to the nucleus and formed subnuclear fine foci. Coexpression of human Runx2 disrupted the AR subnuclear fine foci formation, and the intranuclear fluorescent pattern of AR became similar to that of Runx2. On the other hand, ligand-bound ARs repressed the Runx2-mediated transactivation function. Runx2 was also extracted from its original compartment by ligand-bound ARs. These results suggest that both Runx2 and ARs repress the transactivation function of the other protein by extracting it from its original compartment. The AR and Runx2 may play a mutual role in transcriptional activation in osteoblasts.
|
| Volume |
105(1-5)
|
| Pages |
46-56
|
| Published |
2007-1-1
|
| DOI |
10.1016/j.jsbmb.2006.11.020
|
| PII |
S0960-0760(07)00099-4
|
| PMID |
17627815
|
| MeSH |
3T3 Cells
Animals
Blotting, Western
COS Cells
Cell Compartmentation*
Chlorocebus aethiops
Core Binding Factor Alpha 1 Subunit / genetics*
Green Fluorescent Proteins / metabolism
Mice
Receptors, Androgen / genetics*
Transcriptional Activation*
|
| IF |
3.813
|
| Times Cited |
17
|
|
WOS Category
|
ENDOCRINOLOGY & METABOLISM
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126) |
