| RRC ID |
43042
|
| 著者 |
Kino R, Ikoma T, Yunoki S, Nagai N, Tanaka J, Asakura T, Munekata M.
|
| タイトル |
Preparation and characterization of multilayered hydroxyapatite/silk fibroin film.
|
| ジャーナル |
J Biosci Bioeng
|
| Abstract |
We prepared multilayered films consisting of silk fibroin (SF) and hydroxyapatite (HAp) by alternating lamination using untreated SF and HAp-deposited SF films. Untreated SF films were prepared from a regenerated SF solution by air drying. HAp-deposited SF films were prepared by soaking methanol-treated SF films containing >5 wt% CaCl2 in a simulated body fluid with the ion concentration 1.5-fold higher than that of the standard one. The multilayered HAp/SF films had HAp layers with approximate thicknesses of 3-5 microm and SF layers with thicknesses of 40-70 microm. The bonding strength between the SF and HAp layers was significantly affected by temperature and compression time under the lamination method. The optimal conditions for achieving the maximum T-peel strength and beta-sheet contents were determined to be 130 degrees C for 4 min. The Young's modulus of the multilayered films (133.4 MPa) was higher than that of the films consisting of SF alone (92.5 MPa) under swollen conditions. The biocompatibility of the HAp-deposited SF films was analyzed by culturing of osteoblasts (MC3T3-E1) on a film. The results indicate that HAp-deposited SF films and SF films show similar degrees of cell adhesion and alkaline phosphatase activities.
|
| 巻・号 |
103(6)
|
| ページ |
514-20
|
| 公開日 |
2007-6-1
|
| DOI |
10.1263/jbb.103.514
|
| PII |
S1389-1723(07)70098-1
|
| PMID |
17630122
|
| MeSH |
3T3 Cells
Alkaline Phosphatase / metabolism
Animals
Biocompatible Materials / chemistry*
Biocompatible Materials / isolation & purification
Biomechanical Phenomena
Bombyx / chemistry
Cell Adhesion
Coated Materials, Biocompatible / chemistry
Durapatite / chemistry*
Fibroins / chemistry*
Guided Tissue Regeneration
Membranes, Artificial
Mice
Microscopy, Electron, Scanning
Silk / chemistry*
Tensile Strength
|
| IF |
2.366
|
| 引用数 |
41
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
