論文 - 詳細
| RRC ID | 43042 |
|---|---|
| 著者 | Kino R, Ikoma T, Yunoki S, Nagai N, Tanaka J, Asakura T, Munekata M. |
| タイトル | Preparation and characterization of multilayered hydroxyapatite/silk fibroin film. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
We prepared multilayered films consisting of silk fibroin (SF) and hydroxyapatite (HAp) by alternating lamination using untreated SF and HAp-deposited SF films. Untreated SF films were prepared from a regenerated SF solution by air drying. HAp-deposited SF films were prepared by soaking methanol-treated SF films containing >5 wt% CaCl2 in a simulated body fluid with the ion concentration 1.5-fold higher than that of the standard one. The multilayered HAp/SF films had HAp layers with approximate thicknesses of 3-5 microm and SF layers with thicknesses of 40-70 microm. The bonding strength between the SF and HAp layers was significantly affected by temperature and compression time under the lamination method. The optimal conditions for achieving the maximum T-peel strength and beta-sheet contents were determined to be 130 degrees C for 4 min. The Young's modulus of the multilayered films (133.4 MPa) was higher than that of the films consisting of SF alone (92.5 MPa) under swollen conditions. The biocompatibility of the HAp-deposited SF films was analyzed by culturing of osteoblasts (MC3T3-E1) on a film. The results indicate that HAp-deposited SF films and SF films show similar degrees of cell adhesion and alkaline phosphatase activities. |
| 巻・号 | 103(6) |
| ページ | 514-20 |
| 公開日 | 2007-6-1 |
| DOI | 10.1263/jbb.103.514 |
| PII | S1389-1723(07)70098-1 |
| PMID | 17630122 |
| MeSH | 3T3 Cells Alkaline Phosphatase / metabolism Animals Biocompatible Materials / chemistry* Biocompatible Materials / isolation & purification Biomechanical Phenomena Bombyx / chemistry Cell Adhesion Coated Materials, Biocompatible / chemistry Durapatite / chemistry* Fibroins / chemistry* Guided Tissue Regeneration Membranes, Artificial Mice Microscopy, Electron, Scanning Silk / chemistry* Tensile Strength |
| IF | 2.366 |
| 引用数 | 41 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |