| RRC ID |
43047
|
| Author |
Matsuoka TA, Kaneto H, Stein R, Miyatsuka T, Kawamori D, Henderson E, Kojima I, Matsuhisa M, Hori M, Yamasaki Y.
|
| Title |
MafA regulates expression of genes important to islet beta-cell function.
|
| Journal |
Mol Endocrinol
|
| Abstract |
Insulin transcription factor MafA is unique in being exclusively expressed at the secondary and principal phase of insulin-expressing cell production during pancreas organogenesis and is the only transcriptional activator present exclusively in islet beta-cells. Here we show that ectopic expression of MafA is sufficient to induce a small amount of endogenous insulin expression in a variety of non-beta-cell lines. Insulin mRNA and protein expression was induced to a much higher level when MafA was provided with two other key insulin activators, pancreatic and duodenal homeobox (PDX-1) and BETA2. Potentiation by PDX-1 and BETA2 was entirely dependent upon MafA, and MafA binding to the insulin enhancer region was increased by PDX-1 and BETA2. Treatment with activin A and hepatocyte growth factor induced even larger amounts of insulin in AR42J pancreatic acinar cells, compared with other non-beta endodermal cells. The combination of PDX-1, BETA2, and MafA also induced the expression of other important regulators of islet beta-cell activity. These results support a critical role of MafA in islet beta-cell function.
|
| Volume |
21(11)
|
| Pages |
2764-74
|
| Published |
2007-11-1
|
| DOI |
10.1210/me.2007-0028
|
| PII |
me.2007-0028
|
| PMID |
17636040
|
| MeSH |
Animals
Cell Nucleus / metabolism
Gene Expression Regulation*
Glucose Transporter Type 2 / metabolism
HeLa Cells
Humans
Insulin / metabolism
Insulin-Secreting Cells / metabolism*
Islets of Langerhans / metabolism*
Maf Transcription Factors, Large / metabolism
Maf Transcription Factors, Large / physiology*
Mice
Models, Biological
Protein Binding
RNA, Messenger / metabolism
Transcriptional Activation
|
| IF |
3.628
|
| Times Cited |
71
|
|
WOS Category
|
ENDOCRINOLOGY & METABOLISM
|
| Resource |
| Human and Animal Cells |
IEC 6(RCB0993) |
