RRC ID 43070
Author Simon CG Jr, Stephens JS, Dorsey SM, Becker ML.
Title Fabrication of combinatorial polymer scaffold libraries.
Journal Rev Sci Instrum
Abstract We have designed a novel combinatorial research platform to help accelerate tissue engineering research. Combinatorial methods combine many samples into a single specimen to enable accelerated experimentation and discovery. The platform for fabricating combinatorial polymer scaffold libraries can be used to rapidly identify scaffold formulations that maximize tissue formation. Many approaches for screening cell-biomaterial interactions utilize a two-dimensional format such as a film or surface to present test substrates to cells. However, cells in vivo exist in a three-dimensional milieu of extracellular matrix and cells in vitro behave more naturally when cultured in a three-dimensional environment than when cultured on a two-dimensional surface. Thus, we have designed a method for fabricating combinatorial biomaterial libraries where the materials are presented to cells in the form of three-dimensional, porous, salt-leached, polymer scaffolds. Many scaffold variations and compositions can be screened in a single experiment so that optimal scaffold formulations for tissue formation can be rapidly identified. In summary, we have developed a platform technology for fabricating combinatorial polymer scaffold libraries that can be used to screen cell response to materials in a three-dimensional, scaffold format.
Volume 78(7)
Pages 072207
Published 2007-7-1
DOI 10.1063/1.2755761
PMID 17672738
MeSH Biocompatible Materials / chemistry* Cell Culture Techniques / instrumentation* Cell Culture Techniques / methods Combinatorial Chemistry Techniques / instrumentation* Combinatorial Chemistry Techniques / methods Equipment Design Equipment Failure Analysis Lactic Acid / chemistry* Materials Testing / instrumentation* Materials Testing / methods Microfluidic Analytical Techniques / instrumentation Microfluidic Analytical Techniques / methods Polyesters Polymers / chemistry* Robotics / instrumentation* Robotics / methods Tissue Engineering / instrumentation* Tissue Engineering / methods
IF 1.48
Times Cited 39
Human and Animal Cells MC3T3-E1(RCB1126)