RRC ID 43075
著者 Li TC, Scotti PD, Miyamura T, Takeda N.
タイトル Latent infection of a new alphanodavirus in an insect cell line.
ジャーナル J Virol
Abstract Insect BTI-TN-5B1-4 (Tn5) cells have been used extensively with recombinant baculoviruses to express foreign genes. When a recombinant baculovirus containing the hepatitis E virus capsid protein gene was used to infect Tn5 cells, unknown virus particles in addition to the anticipated hepatitis E virus-like particles were produced in the infected cells. The unknown virus particles were 35 nm in diameter and contained RNA that was highly homologous to full-length RNA1 (3,107 bp) and RNA2 (1,383 bp) genomic RNAs of flock house virus. Surprisingly, both RNAs seen in these induced nodavirus particles could be amplified from commercially available Tn5 cells without infection with or induction by a baculovirus. The nucleotide sequences from the purified nodavirus particles and the normal Tn5 cells were identical, demonstrating that the Tn5 cells themselves were latently infected with a nodavirus. However, the generation of nodavirus particles was significantly stimulated by infection with recombinant baculoviruses. Phylogenetic analysis suggested that this new nodavirus belongs to the genus Alphanodavirus in the family Nodaviridae.
巻・号 81(20)
ページ 10890-6
公開日 2007-10-1
DOI 10.1128/JVI.00807-07
PII JVI.00807-07
PMID 17686877
PMC PMC2045576
MeSH Animals Baculoviridae / genetics Base Sequence Capsid Proteins / genetics Cell Line Hepatitis E virus / chemistry Hepatitis E virus / genetics Insecta Molecular Sequence Data Nodaviridae / genetics* Nodaviridae / isolation & purification* Nodaviridae / pathogenicity Phylogeny RNA Virus Infections* Sequence Analysis, RNA Virion Virus Latency*
IF 4.501
引用数 53
WOS 分野 VIROLOGY
リソース情報
ヒト・動物細胞