論文 - 詳細
| RRC ID | 43075 |
|---|---|
| 著者 | Li TC, Scotti PD, Miyamura T, Takeda N. |
| タイトル | Latent infection of a new alphanodavirus in an insect cell line. |
| ジャーナル | J Virol |
| Abstract |
Insect BTI-TN-5B1-4 (Tn5) cells have been used extensively with recombinant baculoviruses to express foreign genes. When a recombinant baculovirus containing the hepatitis E virus capsid protein gene was used to infect Tn5 cells, unknown virus particles in addition to the anticipated hepatitis E virus-like particles were produced in the infected cells. The unknown virus particles were 35 nm in diameter and contained RNA that was highly homologous to full-length RNA1 (3,107 bp) and RNA2 (1,383 bp) genomic RNAs of flock house virus. Surprisingly, both RNAs seen in these induced nodavirus particles could be amplified from commercially available Tn5 cells without infection with or induction by a baculovirus. The nucleotide sequences from the purified nodavirus particles and the normal Tn5 cells were identical, demonstrating that the Tn5 cells themselves were latently infected with a nodavirus. However, the generation of nodavirus particles was significantly stimulated by infection with recombinant baculoviruses. Phylogenetic analysis suggested that this new nodavirus belongs to the genus Alphanodavirus in the family Nodaviridae. |
| 巻・号 | 81(20) |
| ページ | 10890-6 |
| 公開日 | 2007-10-1 |
| DOI | 10.1128/JVI.00807-07 |
| PII | JVI.00807-07 |
| PMID | 17686877 |
| PMC | PMC2045576 |
| MeSH | Animals Baculoviridae / genetics Base Sequence Capsid Proteins / genetics Cell Line Hepatitis E virus / chemistry Hepatitis E virus / genetics Insecta Molecular Sequence Data Nodaviridae / genetics* Nodaviridae / isolation & purification* Nodaviridae / pathogenicity Phylogeny RNA Virus Infections* Sequence Analysis, RNA Virion Virus Latency* |
| IF | 4.501 |
| 引用数 | 53 |
| WOS 分野 | VIROLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Sf9(RCB0563) |