RRC ID 43089
Author Besson N, Hullin-Matsuda F, Makino A, Murate M, Lagarde M, Pageaux JF, Kobayashi T, Delton-Vandenbroucke I.
Title Selective incorporation of docosahexaenoic acid into lysobisphosphatidic acid in cultured THP-1 macrophages.
Journal Lipids
Abstract Lysobisphosphatidic acid (LBPA) is highly accumulated in specific domains of the late endosome and is involved in the biogenesis and function of this organelle. Little is known about the biosynthesis and metabolism of this lipid. We examined its FA composition and the incorporation of exogenous FA into LBPA in the human monocytic leukemia cell line THP-1. The LBPA FA composition in THP-1 cells exhibits an elevated amount of oleic acid (18:1n-9) and enrichment of PUFA, especially DHA (22:6n-3). DHA supplemented to the medium was efficiently incorporated into LBPA. In contrast, arachidonic acid (20:4n-6) was hardly esterified to LBPA under the same experimental conditions. The turnover of DHA in LBPA was similar to that in other phospholipids. Specific incorporation of DHA into LBPA was also observed in baby hamster kidney fibroblasts, although LBPA in these cells contains very low endogenous levels of DHA in normal growth conditions. Our resuIts, together with published observations, suggest that the specific incorporation of DHA into LBPA is a common phenomenon in mammalian cells. The physiological significance of DHA-enriched LBPA is discussed.
Volume 41(2)
Pages 189-96
Published 2006-2
DOI 10.1007/s11745-006-5087-5
PMID 17707985
MeSH Animals Arachidonic Acid / metabolism Cell Line, Tumor Cricetinae Docosahexaenoic Acids / metabolism* Endosomes / metabolism Humans Lysophospholipids / biosynthesis* Macrophages / metabolism Monoglycerides / biosynthesis* Phosphatidylcholines / metabolism Phosphatidylethanolamines / metabolism
IF 1.936
Times Cited 11
Human and Animal Cells