論文 - 詳細
| RRC ID | 43090 |
|---|---|
| 著者 | Zhang Y, Li Y, Shibahara S, Takahashi K. |
| タイトル | Synergistic activation of the human adrenomedullin gene promoter by Sp1 and AP-2alpha. |
| ジャーナル | Peptides |
| Abstract |
Adrenomedullin (AM) is a potent vasodilator peptide, which is ubiquitously expressed and has various biological actions, such as proliferative action and anti-oxidative stress action. AM expression is induced by various stresses, such as hypoxia and inflammatory cytokines, and during cell differentiation. The human AM gene promoter region (-70/-29) contains binding sites for stimulatory protein 1 (Sp1) and activator protein-2alpha (AP-2alpha), and has been shown to be important for the AM gene expression during cell differentiation to macrophages or adipocytes. We here show that Sp1 and AP-2alpha synergistically activate the AM gene promoter. Co-transfection of the reporter plasmid containing the AM promoter region (-103/-29) with Sp1 and AP-2alpha expression plasmids showed that Sp1 and AP-2alpha synergistically increased the promoter activity in HeLa cells. Sp1 or AP-2alpha alone caused only small increases in the promoter activity. EMSA showed that Sp1 bound to the promoter region (-70/-29), whereas AP-2alpha bound to a more upstream promoter region (-103/-71). Thus, the synergistic activation of the human AM gene promoter by Sp1 and AP-2alpha may be mediated by the binding of Sp1 to the promoter region (-70/-29) and the interaction with AP-2alpha, which binds to the promoter region (-103/-71). |
| 巻・号 | 29(3) |
| ページ | 465-72 |
| 公開日 | 2008-3-1 |
| DOI | 10.1016/j.peptides.2007.07.015 |
| PII | S0196-9781(07)00236-7 |
| PMID | 17719138 |
| MeSH | Adrenomedullin / genetics* Binding Sites / genetics Electrophoretic Mobility Shift Assay Gene Expression Regulation HeLa Cells Humans Promoter Regions, Genetic / genetics* Protein Binding Sp1 Transcription Factor / genetics Sp1 Transcription Factor / metabolism* Transcription Factor AP-2 / genetics Transcription Factor AP-2 / metabolism* Transfection |
| IF | 2.843 |
| 引用数 | 5 |
| WOS 分野 | PHARMACOLOGY & PHARMACY ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |