RRC ID 43092
Author Hacchou Y, Uematsu T, Ueda O, Usui Y, Uematsu S, Takahashi M, Uchihashi T, Kawazoe Y, Shiba T, Kurihara S, Yamaoka M, Furusawa K.
Title Inorganic polyphosphate: a possible stimulant of bone formation.
Journal J Dent Res
Abstract Inorganic polyphosphates [Poly(P)] are often distributed in osteoblasts. We undertook the present study to verify the hypothesis that Poly(P) stimulates osteoblasts and facilitates bone formation. The osteoblast-like cell line MC 3T3-E1 was cultured with Poly(P), and gene expression and potential mineralization were evaluated by reverse-transcription polymerase chain-reaction. Alkaline phosphatase activity, von Kossa staining, and resorption pit formation analyses were also determined. The potential role of Poly(P) in bone formation was assessed in a rat alveolar bone regeneration model. Poly(P) induced osteopontin, osteocalcin, collagen 1alpha, and osteoprotegerin expression and increased alkaline phosphatase activity in MC 3T3-E1 cells. Dentin slice pit formation decreased with mouse osteoblast and bone marrow macrophage co-cultivation in the presence of Poly(P). Promotion of alveolar bone regeneration was observed locally in Poly(P)-treated rats. These findings suggest that Poly(P) plays a role in osteoblastic differentiation, activation, and bone mineralization. Thus, local poly(P) delivery may have a therapeutic benefit in periodontal disease.
Volume 86(9)
Pages 893-7
Published 2007-9-1
DOI 10.1177/154405910708600917
PII 86/9/893
PMID 17720862
MeSH 3T3 Cells Alveolar Bone Loss / drug therapy* Animals Bone Regeneration / drug effects Cell Differentiation / drug effects Coculture Techniques Collagen Type I / biosynthesis Macrophages Male Mice Osteoblasts / drug effects* Osteoblasts / metabolism Osteocalcin / biosynthesis Osteoclasts / drug effects Osteogenesis / drug effects* Osteopontin / biosynthesis Osteoprotegerin / biosynthesis Phosphates / pharmacology* Phosphates / therapeutic use Polyphosphates / pharmacology* Polyphosphates / therapeutic use Rats Rats, Wistar
IF 4.914
Times Cited 49
Human and Animal Cells MC3T3-E1(RCB1126)