| RRC ID |
43092
|
| Author |
Hacchou Y, Uematsu T, Ueda O, Usui Y, Uematsu S, Takahashi M, Uchihashi T, Kawazoe Y, Shiba T, Kurihara S, Yamaoka M, Furusawa K.
|
| Title |
Inorganic polyphosphate: a possible stimulant of bone formation.
|
| Journal |
J Dent Res
|
| Abstract |
Inorganic polyphosphates [Poly(P)] are often distributed in osteoblasts. We undertook the present study to verify the hypothesis that Poly(P) stimulates osteoblasts and facilitates bone formation. The osteoblast-like cell line MC 3T3-E1 was cultured with Poly(P), and gene expression and potential mineralization were evaluated by reverse-transcription polymerase chain-reaction. Alkaline phosphatase activity, von Kossa staining, and resorption pit formation analyses were also determined. The potential role of Poly(P) in bone formation was assessed in a rat alveolar bone regeneration model. Poly(P) induced osteopontin, osteocalcin, collagen 1alpha, and osteoprotegerin expression and increased alkaline phosphatase activity in MC 3T3-E1 cells. Dentin slice pit formation decreased with mouse osteoblast and bone marrow macrophage co-cultivation in the presence of Poly(P). Promotion of alveolar bone regeneration was observed locally in Poly(P)-treated rats. These findings suggest that Poly(P) plays a role in osteoblastic differentiation, activation, and bone mineralization. Thus, local poly(P) delivery may have a therapeutic benefit in periodontal disease.
|
| Volume |
86(9)
|
| Pages |
893-7
|
| Published |
2007-9-1
|
| DOI |
10.1177/154405910708600917
|
| PII |
86/9/893
|
| PMID |
17720862
|
| MeSH |
3T3 Cells
Alveolar Bone Loss / drug therapy*
Animals
Bone Regeneration / drug effects
Cell Differentiation / drug effects
Coculture Techniques
Collagen Type I / biosynthesis
Macrophages
Male
Mice
Osteoblasts / drug effects*
Osteoblasts / metabolism
Osteocalcin / biosynthesis
Osteoclasts / drug effects
Osteogenesis / drug effects*
Osteopontin / biosynthesis
Osteoprotegerin / biosynthesis
Phosphates / pharmacology*
Phosphates / therapeutic use
Polyphosphates / pharmacology*
Polyphosphates / therapeutic use
Rats
Rats, Wistar
|
| IF |
4.914
|
| Times Cited |
49
|
|
WOS Category
|
DENTISTRY, ORAL SURGERY & MEDICINE
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126) |
