論文 - 詳細
| RRC ID | 43092 |
|---|---|
| 著者 | Hacchou Y, Uematsu T, Ueda O, Usui Y, Uematsu S, Takahashi M, Uchihashi T, Kawazoe Y, Shiba T, Kurihara S, Yamaoka M, Furusawa K. |
| タイトル | Inorganic polyphosphate: a possible stimulant of bone formation. |
| ジャーナル | J Dent Res |
| Abstract |
Inorganic polyphosphates [Poly(P)] are often distributed in osteoblasts. We undertook the present study to verify the hypothesis that Poly(P) stimulates osteoblasts and facilitates bone formation. The osteoblast-like cell line MC 3T3-E1 was cultured with Poly(P), and gene expression and potential mineralization were evaluated by reverse-transcription polymerase chain-reaction. Alkaline phosphatase activity, von Kossa staining, and resorption pit formation analyses were also determined. The potential role of Poly(P) in bone formation was assessed in a rat alveolar bone regeneration model. Poly(P) induced osteopontin, osteocalcin, collagen 1alpha, and osteoprotegerin expression and increased alkaline phosphatase activity in MC 3T3-E1 cells. Dentin slice pit formation decreased with mouse osteoblast and bone marrow macrophage co-cultivation in the presence of Poly(P). Promotion of alveolar bone regeneration was observed locally in Poly(P)-treated rats. These findings suggest that Poly(P) plays a role in osteoblastic differentiation, activation, and bone mineralization. Thus, local poly(P) delivery may have a therapeutic benefit in periodontal disease. |
| 巻・号 | 86(9) |
| ページ | 893-7 |
| 公開日 | 2007-9-1 |
| DOI | 10.1177/154405910708600917 |
| PII | 86/9/893 |
| PMID | 17720862 |
| MeSH | 3T3 Cells Alveolar Bone Loss / drug therapy* Animals Bone Regeneration / drug effects Cell Differentiation / drug effects Coculture Techniques Collagen Type I / biosynthesis Macrophages Male Mice Osteoblasts / drug effects* Osteoblasts / metabolism Osteocalcin / biosynthesis Osteoclasts / drug effects Osteogenesis / drug effects* Osteopontin / biosynthesis Osteoprotegerin / biosynthesis Phosphates / pharmacology* Phosphates / therapeutic use Polyphosphates / pharmacology* Polyphosphates / therapeutic use Rats Rats, Wistar |
| IF | 4.914 |
| 引用数 | 49 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |