| RRC ID |
43109
|
| Author |
Masuda S, Yamamoto K, Hirabayashi T, Ishikawa Y, Ishii T, Kudo I, Murakami M.
|
| Title |
Human group III secreted phospholipase A2 promotes neuronal outgrowth and survival.
|
| Journal |
Biochem J
|
| Abstract |
Human sPLA2-III [group III secreted PLA2 (phospholipase A2)] is an atypical sPLA2 isoenzyme that consists of a central group III sPLA2 domain flanked by unique N- and C-terminal domains. In the present study, we found that sPLA2-III is expressed in neuronal cells, such as peripheral neuronal fibres, spinal DRG (dorsal root ganglia) neurons and cerebellar Purkinje cells. Adenoviral expression of sPLA2-III in PC12 cells (pheochromocytoma cells) or DRG explants facilitated neurite outgrowth, whereas expression of a catalytically inactive sPLA2-III mutant or use of sPLA2-III-directed siRNA (small interfering RNA) reduced NGF (nerve growth factor)-induced neuritogenesis. sPLA2-III also suppressed neuronal death induced by NGF deprivation. Lipid MS revealed that sPLA2-III overexpression increased the cellular level of lysophosphatidylcholine, a PLA2 reaction product with neuritogenic and neurotropic activities, whereas siRNA knockdown reduced the level of lysophosphatidylcholine. These observations suggest the potential contribution of sPLA2-III to neuronal differentiation and its function under certain conditions.
|
| Volume |
409(2)
|
| Pages |
429-38
|
| Published |
2008-1-15
|
| DOI |
10.1042/BJ20070844
|
| PII |
BJ20070844
|
| PMID |
17868035
|
| MeSH |
Animals
Cell Differentiation
Cell Survival / radiation effects
DNA, Complementary / metabolism
Group III Phospholipases A2 / metabolism*
Humans
Mice
Mice, Inbred C57BL
Nerve Growth Factor / metabolism
Neurites / physiology
Neurons / cytology*
Neurons / enzymology*
PC12 Cells
RNA, Small Interfering / metabolism
Rats
Spectrometry, Mass, Electrospray Ionization
|
| IF |
4.097
|
| Times Cited |
40
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
PC-12(RCB0009) |
