論文 - 詳細
| RRC ID | 43112 |
|---|---|
| 著者 | Yanagawa T, Funasaka T, Tsutsumi S, Hu H, Watanabe H, Raz A. |
| タイトル | Regulation of phosphoglucose isomerase/autocrine motility factor activities by the poly(ADP-ribose) polymerase family-14. |
| ジャーナル | Cancer Res |
| Abstract |
Phosphoglucose isomerase (PGI; EC 5.3.1.9) is a ubiquitous cytosolic enzyme essential for glycolysis and gluconeogenesis. PGI is a multifunctional dimeric protein that extracellularly acts as a cytokine [autocrine motility factor (AMF)] eliciting mitogenic, motogenic, and differentiation functions through binding to its cell surface receptor gp78/AMF receptor (AMFR). AMFR contains a seven-transmembrane domain with RING-H2 and leucine zipper motifs showing ubiquitin protein ligase (E3) activity and is exposed on the endoplasmic reticulum surface. Augmented expressions of both PGI/AMF and AMFR have been implicated in tumor progression and metastasis, and an intracellular binding partner of PGI/AMF is expected to regulate in part its diverse biological functions. Thus, we screened a cDNA library using a yeast two-hybrid system to search for interacting protein(s) and report on the finding of poly(ADP-ribose) polymerase-14 (PARP-14) to be a binding partner with PGI/AMF. PARP-14-PGI/AMF interaction was confirmed by coimmunoprecipitation and immunolocalization. We also report that PGI/AMF degradation is mainly regulated by the ubiquitin-lysosome system and RNA interference experiments revealed that PARP-14 inhibits PGI/AMF ubiquitination, thus contributing to its stabilization and secretion. This newly characterized PARP-14 protein should assist in understanding the regulation of PGI/AMF intracellular function(s) and may provide a new therapeutic target for inhibition of PGI/AMF inducing tumor cell migration and invasion during metastasis. |
| 巻・号 | 67(18) |
| ページ | 8682-9 |
| 公開日 | 2007-9-15 |
| DOI | 10.1158/0008-5472.CAN-07-1586 |
| PII | 67/18/8682 |
| PMID | 17875708 |
| MeSH | Bone Neoplasms / enzymology Colonic Neoplasms / enzymology DNA, Complementary / genetics DNA, Complementary / isolation & purification Endothelial Cells / enzymology Fibrosarcoma / enzymology Glucose-6-Phosphate Isomerase / metabolism* HT29 Cells Humans Osteosarcoma / enzymology Poly(ADP-ribose) Polymerases / genetics Poly(ADP-ribose) Polymerases / metabolism* |
| IF | 9.727 |
| 引用数 | 27 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MKN45(RCB1001) |