| RRC ID |
43112
|
| 著者 |
Yanagawa T, Funasaka T, Tsutsumi S, Hu H, Watanabe H, Raz A.
|
| タイトル |
Regulation of phosphoglucose isomerase/autocrine motility factor activities by the poly(ADP-ribose) polymerase family-14.
|
| ジャーナル |
Cancer Res
|
| Abstract |
Phosphoglucose isomerase (PGI; EC 5.3.1.9) is a ubiquitous cytosolic enzyme essential for glycolysis and gluconeogenesis. PGI is a multifunctional dimeric protein that extracellularly acts as a cytokine [autocrine motility factor (AMF)] eliciting mitogenic, motogenic, and differentiation functions through binding to its cell surface receptor gp78/AMF receptor (AMFR). AMFR contains a seven-transmembrane domain with RING-H2 and leucine zipper motifs showing ubiquitin protein ligase (E3) activity and is exposed on the endoplasmic reticulum surface. Augmented expressions of both PGI/AMF and AMFR have been implicated in tumor progression and metastasis, and an intracellular binding partner of PGI/AMF is expected to regulate in part its diverse biological functions. Thus, we screened a cDNA library using a yeast two-hybrid system to search for interacting protein(s) and report on the finding of poly(ADP-ribose) polymerase-14 (PARP-14) to be a binding partner with PGI/AMF. PARP-14-PGI/AMF interaction was confirmed by coimmunoprecipitation and immunolocalization. We also report that PGI/AMF degradation is mainly regulated by the ubiquitin-lysosome system and RNA interference experiments revealed that PARP-14 inhibits PGI/AMF ubiquitination, thus contributing to its stabilization and secretion. This newly characterized PARP-14 protein should assist in understanding the regulation of PGI/AMF intracellular function(s) and may provide a new therapeutic target for inhibition of PGI/AMF inducing tumor cell migration and invasion during metastasis.
|
| 巻・号 |
67(18)
|
| ページ |
8682-9
|
| 公開日 |
2007-9-15
|
| DOI |
10.1158/0008-5472.CAN-07-1586
|
| PII |
67/18/8682
|
| PMID |
17875708
|
| MeSH |
Bone Neoplasms / enzymology
Colonic Neoplasms / enzymology
DNA, Complementary / genetics
DNA, Complementary / isolation & purification
Endothelial Cells / enzymology
Fibrosarcoma / enzymology
Glucose-6-Phosphate Isomerase / metabolism*
HT29 Cells
Humans
Osteosarcoma / enzymology
Poly(ADP-ribose) Polymerases / genetics
Poly(ADP-ribose) Polymerases / metabolism*
|
| IF |
9.727
|
| 引用数 |
27
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MKN45(RCB1001) |
