| RRC ID |
43116
|
| 著者 |
Sanuki R, Mitsui N, Suzuki N, Koyama Y, Yamaguchi A, Isokawa K, Shimizu N, Maeno M.
|
| タイトル |
Effect of compressive force on the production of prostaglandin E(2) and its receptors in osteoblastic Saos-2 cells.
|
| ジャーナル |
Connect Tissue Res
|
| Abstract |
In orthodontic tooth movement, prostaglandin E(2) (PGE(2)) released from osteoblasts can alter the normal process of bone remodeling. We examined the effect of compressive force (CF) on PGE(2) production, PGE receptors (Ep1-4) expression, phosphorylation of protein kinase A (p-PKA), and calcium content in Saos-2 cells. PGE(2) production increased as CF strength. Applying CF of 98 or 294 Pa caused the cells to produce approximately 700 and 1,400 pg/mL PGE(2), respectively. CF of 98 Pa increased Ep2 gene expression, and 98 and 294 Pa CF increased Ep4. Immunohistochemical staining showed strong expression of Ep2 under 98 Pa and Ep4 under 98 and 294 Pa. The p-PKA increased as the strength of CF or PGE(2) concentration. The calcium content was increased by the addition of 700 pg/mL PGE(2) but was decreased by 1400 pg/mL. Thus, mechanical stress controls bone formation by stimulating PGE(2) production and Ep2 and/or Ep4 expression in osteoblasts.
|
| 巻・号 |
48(5)
|
| ページ |
246-53
|
| 公開日 |
2007-1-1
|
| DOI |
10.1080/03008200701541775
|
| PII |
782091740
|
| PMID |
17882700
|
| MeSH |
Calcium / analysis
Cell Line, Tumor
Cyclic AMP-Dependent Protein Kinases / metabolism*
Dinoprostone / biosynthesis*
Humans
Osteoblasts / cytology
Osteoblasts / enzymology
Osteoblasts / metabolism*
Phosphorylation
Receptors, Prostaglandin E / biosynthesis*
Receptors, Prostaglandin E / isolation & purification
Stress, Mechanical
|
| IF |
2.795
|
| 引用数 |
16
|
|
WOS 分野
|
ORTHOPEDICS
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Saos-2(RCB0428) |
