RRC ID 4312
Author Vázquez-Manrique RP, Nagy AI, Legg JC, Bales OA, Ly S, Baylis HA.
Title Phospholipase C-epsilon regulates epidermal morphogenesis in Caenorhabditis elegans.
Journal PLoS Genet
Abstract Migration of cells within epithelial sheets is an important feature of embryogenesis and other biological processes. Previous work has demonstrated a role for inositol 1,4,5-trisphosphate (IP(3))-mediated calcium signalling in the rearrangement of epidermal cells (also known as hypodermal cells) during embryonic morphogenesis in Caenorhabditis elegans. However the mechanism by which IP(3) production is stimulated is unknown. IP(3) is produced by the action of phospholipase C (PLC). We therefore surveyed the PLC family of C. elegans using RNAi and mutant strains, and found that depletion of PLC-1/PLC-epsilon produced substantial embryonic lethality. We used the epithelial cell marker ajm-1::gfp to follow the behaviour of epidermal cells and found that 96% of the arrested embryos have morphogenetic defects. These defects include defective ventral enclosure and aberrant dorsal intercalation. Using time-lapse confocal microscopy we show that the migration of the ventral epidermal cells, especially of the leading cells, is slower and often fails in plc-1(tm753) embryos. As a consequence plc-1 loss of function results in ruptured embryos with a Gex phenotype (gut on exterior) and lumpy larvae. Thus PLC-1 is involved in the regulation of morphogenesis. Genetic studies using gain- and loss-of-function alleles of itr-1, the gene encoding the IP(3) receptor in C. elegans, demonstrate that PLC-1 acts through ITR-1. Using RNAi and double mutants to deplete the other PLCs in a plc-1 background, we show that PLC-3/PLC-gamma and EGL-8/PLC-beta can compensate for reduced PLC-1 activity. Our work places PLC-epsilon into a pathway controlling epidermal cell migration, thus establishing a novel role for PLC-epsilon.
Volume 4(3)
Pages e1000043
Published 2008-3-28
DOI 10.1371/journal.pgen.1000043
PMID 18369461
PMC PMC2274882
MeSH Animals Animals, Genetically Modified Base Sequence Caenorhabditis elegans / embryology* Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / antagonists & inhibitors Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* DNA Primers / genetics DNA, Helminth / genetics Embryonic Development / genetics Epidermis / embryology Epidermis / enzymology Female Gene Deletion Genes, Helminth Inositol 1,4,5-Trisphosphate / metabolism Morphogenesis Ovulation / genetics Phosphoinositide Phospholipase C / antagonists & inhibitors Phosphoinositide Phospholipase C / genetics Phosphoinositide Phospholipase C / physiology* RNA Interference Signal Transduction
IF 5.224
Times Cited 24
WOS Category GENETICS & HEREDITY
Resource
C.elegans tm753 tm738 tm1340 tm1304