RRC ID 43129
Author Hirai S, Kim YI, Goto T, Kang MS, Yoshimura M, Obata A, Yu R, Kawada T.
Title Inhibitory effect of naringenin chalcone on inflammatory changes in the interaction between adipocytes and macrophages.
Journal Life Sci
Abstract Obese adipose tissue is characterized by an enhanced infiltration of macrophages. It is considered that the paracrine loop involving monocyte chemoattractant protein (MCP)-1 and tumor necrosis factor (TNF)-alpha between adipocytes and macrophages establishes a vicious cycle that augments the inflammatory changes and insulin resistance in obese adipose tissue. Polyphenols, which are widely distributed in fruit and vegetables, can act as antioxidants and some of them are also reported to have anti-inflammatory properties. Tomato is one of the most popular and extensively consumed vegetable crops worldwide, which also contains many flavonoids, mainly naringenin chalcone. We investigated the effect of flavonoids, including naringenin chalcone, on the production of proinflammatory mediators in lipopolysaccharide (LPS)-stimulated macrophages and in the interaction between adipocytes and macrophages. Naringenin chalcone inhibited the production of TNF-alpha, MCP-1, and nitric oxide (NO) by LPS-stimulated RAW 264 macrophages in a dose-dependent manner. Coculture of 3T3-L1 adipocytes and RAW 264 macrophages markedly enhanced the production of TNF-alpha, MCP-1, and NO compared with the control cultures; however, treatment with naringenin chalcone dose-dependently inhibited the production of these proinflammatory mediators. These results indicate that naringenin chalcone exhibits anti-inflammatory properties by inhibiting the production of proinflammatory cytokines in the interaction between adipocytes and macrophages. Naringenin chalcone may be useful for ameliorating the inflammatory changes in obese adipose tissue.
Volume 81(16)
Pages 1272-9
Published 2007-9-29
DOI 10.1016/j.lfs.2007.09.001
PII S0024-3205(07)00647-9
PMID 17915259
MeSH 3T3-L1 Cells Adipocytes* / cytology Adipocytes* / drug effects Adipocytes* / immunology Animals Blotting, Western Cell Communication / drug effects* Cell Communication / immunology Cell Survival / drug effects Chalcones / metabolism Chalcones / pharmacology* Chemokine CCL2 / biosynthesis Chemokine CCL2 / immunology Cytokines / biosynthesis Cytokines / immunology* I-kappa B Proteins / biosynthesis Lipopolysaccharides / pharmacology Macrophage Activation / drug effects* Macrophage Activation / immunology Macrophages* / cytology Macrophages* / drug effects Macrophages* / immunology Mice NF-KappaB Inhibitor alpha Nitric Oxide / metabolism Nitric Oxide Synthase Type II / biosynthesis Tumor Necrosis Factor-alpha / biosynthesis Tumor Necrosis Factor-alpha / immunology
IF 3.448
Times Cited 95
Human and Animal Cells