| RRC ID |
43135
|
| 著者 |
Akahira J, Suzuki F, Suzuki T, Miura I, Kamogawa N, Miki Y, Ito K, Yaegashi N, Sasano H.
|
| タイトル |
Decreased expression of RIZ1 and its clinicopathological significance in epithelial ovarian carcinoma: correlation with epigenetic inactivation by aberrant DNA methylation.
|
| ジャーナル |
Pathol Int
|
| Abstract |
The retinoblastoma protein-interacting zinc finger gene (RIZ1) is considered a tumor suppressor gene. The purpose of the present study was to examine the expression of RIZ1 and evaluate its clinicopathological significance in ovarian carcinoma. Immunohistochemistry and quantitative reverse transcription-polymerase chain reaction (RT-PCR) was performed for RIZ1 and its clinicopathological significance was examined. DNA methylation status of RIZ1 was also studied. All (6/6) of the normal, 5/9 of benign, and 4/9 of borderline tissues were positive for RIZ1 protein. In ovarian cancer tissues 32.9% (54/164) were positive for RIZ1. Decreased expression of RIZ1 was significantly correlated with histological subtypes (P < 0.0001), high tumor grade (P = 0.0153) and advanced clinical stage (P = 0.0345), and high Ki67 index (P = 0.0117) but was not associated with the overall prognoses of the patients (P = 0.519). The presence of methylated band was detected in 2/9 cell lines, and 5/69 ovarian cancer tissues. Median values of relative RIZ1 expression in cell lines with methylation were significantly lower than those without methylation (P = 0.0404), and treatment of 5-aza-2'deoxycitidine resulted in demethylation and re-expression of RIZ1. Reduced expression of RIZ1 may play an important role in the pathogenesis and/or development of epithelial ovarian carcinoma, and is considered to be caused in part by aberrant DNA methylation.
|
| 巻・号 |
57(11)
|
| ページ |
725-33
|
| 公開日 |
2007-11-1
|
| DOI |
10.1111/j.1440-1827.2007.02169.x
|
| PII |
PIN2169
|
| PMID |
17922684
|
| MeSH |
Carcinoma / genetics*
Carcinoma / metabolism*
Carcinoma / pathology
Cell Line, Tumor
DNA Methylation
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / metabolism*
Epigenesis, Genetic
Female
Histone-Lysine N-Methyltransferase
Humans
Immunohistochemistry
Middle Aged
Neoplasm Staging
Nuclear Proteins / genetics*
Nuclear Proteins / metabolism*
Ovarian Neoplasms / genetics*
Ovarian Neoplasms / metabolism*
Ovarian Neoplasms / pathology
Promoter Regions, Genetic / genetics
Reverse Transcriptase Polymerase Chain Reaction
Transcription Factors / genetics*
Transcription Factors / metabolism*
|
| IF |
2.11
|
| 引用数 |
11
|
|
WOS 分野
|
PATHOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
