Reference - Detail
RRC ID | 43150 |
---|---|
Author | Takahashi N, Udagawa N, Kobayashi Y, Suda T. |
Title | Generation of osteoclasts in vitro, and assay of osteoclast activity. |
Journal | Methods Mol Med |
Abstract |
Osteoclasts are bone-resorbing multinucleated cells derived from the monocyte-macrophage lineage. The authors have developed a mouse marrow culture system and a coculture system of mouse osteoblasts and hemopoietic cells, in which osteoclasts are formed in response to various osteotropic factors such as 1alpha,25-dihydroxyvitamin D3, parathyroid hormone, prostaglandin E2, and interleukin-11. Recent studies have revealed that osteoblasts express two cytokines essential for osteoclastogenesis: receptor activator of nuclear factor kappaB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). Using RANKL and M-CSF, we can induce osteoclasts from monocyte-macrophage lineage cells even in the absence of osteoblasts. This chapter describes the methods for osteoclast formation in vitro in the presence and absence of osteoblasts, and for pit-formation assay using dentine slices and osteoclasts formed in vitro. These culture systems have made it possible to investigate each step of osteoclast development and function separately. |
Volume | 135 |
Pages | 285-301 |
Published | 2007-1-1 |
DOI | 10.1007/978-1-59745-401-8_18 |
PII | 1-59745-401-X:285 |
PMID | 17951666 |
MeSH | Actins / metabolism Animals Bone Marrow Cells / cytology Bone Resorption / metabolism Bone Resorption / pathology Buffers Cathepsin K Cathepsins / metabolism Cell Differentiation / drug effects Cell Line Cell Separation / methods Coculture Techniques / methods Collagen Culture Media Gels Immunohistochemistry / methods Macrophage Colony-Stimulating Factor / metabolism Macrophage Colony-Stimulating Factor / pharmacology Macrophages / cytology Mice Models, Biological Osteoblasts / cytology Osteoclasts / cytology* Osteoclasts / drug effects Osteoclasts / metabolism RANK Ligand / metabolism RANK Ligand / pharmacology |
Resource | |
Human and Animal Cells | RAW 264(RCB0535) ST2(RCB0224) MC3T3-G2/PA6(RCB1127) |