RRC ID 43151
著者 Nakatani T, Honda E, Hayakawa S, Sato M, Satoh K, Kudo M, Munakata H.
タイトル Effects of decorin on the expression of alpha-smooth muscle actin in a human myofibroblast cell line.
ジャーナル Mol Cell Biochem
Abstract Myofibroblasts are metabolically and morphologically distinctive fibroblasts expressing alpha-smooth muscle actin (alpha-SMA), and their activation plays a key role in development of the fibrotic response. In an activated state, myofibroblasts cease to proliferate and start to synthesize large amounts of extracellular component proteins. The expression of alpha-SMA correlates with the activation of myofibroblasts. Decorin, a member of the small leucine-rich proteoglycan gene family, has been implicated in the negative control of cell proliferation primarily by upregulating the expression of p21, a potent inhibitor of cyclin-dependent kinase. In order to examine the effect of decorin on myofibroblast cell growth, we rendered a human lung myofibroblast cell line, MRC-5, quiescent by either cell-cell contact or serum starvation, and examined the relationship between decorin and alpha-SMA expression in these cells. The expression of decorin in cells made quiescent by serum starvation was lower than that in cells made quiescent by cell-cell contact. In contrast, the expression of alpha-SMA in cells made quiescent by cell-cell contact was lower than that in cells made quiescent by serum starvation. Furthermore, forced expression of decorin was accompanied by a suppression of alpha-SMA expression, whereas knocking down of decorin expression by RNA interference increased the expression of alpha-SMA.
巻・号 308(1-2)
ページ 201-7
公開日 2008-1-1
DOI 10.1007/s11010-007-9629-9
PMID 17952560
MeSH Actins / metabolism* Blotting, Western Cell Line Cell Proliferation Decorin Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / metabolism* Fibroblasts / cytology* Fibroblasts / metabolism* Gene Expression Regulation Humans Muscle, Smooth / metabolism* Proteoglycans / genetics Proteoglycans / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Vimentin / metabolism
IF 2.795
引用数 10
WOS 分野 CELL BIOLOGY
リソース情報
ヒト・動物細胞