RRC ID 43164
Author Wu WK, Li GR, Wong TM, Wang JY, Yu L, Cho CH.
Title Involvement of voltage-gated K+ and Na+ channels in gastric epithelial cell migration.
Journal Mol. Cell. Biochem.
Abstract Epithelial cell migration plays an important role in gastrointestinal mucosal repair. We previously reported that multiple functional ion channels, including a Ba(2+)-sensitive K(+) inward rectifier K(ir)1.2, 4-aminopyridine (4-AP)-sensitive voltage-gated K(+) channels K(v)1.1, K(v)1.6 and K(v)2.1, and a nifedipine-sensitive, tetrodotoxin (TTX)-insensitive voltage-gated Na(+) channel Na(v)1.5 were expressed in a non-transformed rat gastric epithelial cell line (RGM-1). In the present study, we further investigated whether these ion channels are involved in the modulation of gastric epithelial cell migration. Cell migration was determined by monolayer wound healing assay. Results showed that blockade of K(v) with 4-AP or Na(v)1.5 with nifedipine inhibited RGM-1 cell migration in the absence or presence of epidermal growth factor (EGF), which effectively stimulated RGM-1 cell migration. Moreover, high concentration of TTX mimicked the action of nifedipine, suggesting that the action of nifedipine was mediated through specific blockade of Na(v)1.5. In contrast, inhibition of K(ir)1.2 with Ba(2+), either in basal or EGF-stimulated condition, had no effect on RGM-1 cell migration. In conclusion, the present study demonstrates for the first time that voltage-gated K(+) and Na(+) channels are involved in the modulation of gastric epithelial cell migration.
Volume 308(1-2)
Pages 219-26
Published 2008-1
DOI 10.1007/s11010-007-9631-2
PMID 17978865
MeSH 4-Aminopyridine / pharmacology Animals Barium / pharmacology Cell Line Cell Movement* / drug effects Epidermal Growth Factor / pharmacology Epithelial Cells / cytology* Epithelial Cells / drug effects Epithelial Cells / metabolism Gastric Mucosa / cytology* Gastric Mucosa / drug effects Gastric Mucosa / metabolism Gene Expression Regulation / drug effects Ion Channel Gating* / drug effects Nifedipine / pharmacology Potassium Channel Blockers / pharmacology Potassium Channels, Voltage-Gated / genetics Potassium Channels, Voltage-Gated / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism Rats Sodium Channel Blockers / pharmacology Sodium Channels / genetics Sodium Channels / metabolism* Tetrodotoxin / pharmacology
IF 2.561
Times Cited 8
Human and Animal Cells