| RRC ID |
43185
|
| Author |
Kobayashi K, Tada K, Itabe H, Ueno T, Liu PH, Tsutsumi A, Kuwana M, Yasuda T, Shoenfeld Y, de Groot PG, Matsuura E.
|
| Title |
Distinguished effects of antiphospholipid antibodies and anti-oxidized LDL antibodies on oxidized LDL uptake by macrophages.
|
| Journal |
Lupus
|
| Abstract |
Several interpretations have been made regarding the specificity of antiphospholipid antibodies and antibodies against oxidized low-density lipoprotein (oxLDL), but these are still controversial. In the present study, we delineated specificity of these two types of antibodies and analyzed their regulatory effect on oxLDL and/or beta( 2)-glycoprotein I (beta(2)GPI) binding to macrophages. Scavenger receptor-mediated binding of oxLDL (or its beta(2)GPI complexes) to macrophages was observed and the binding was partly prevented by beta( 2)GPI. The IgG monoclonal anti-beta(2)GPI antibody (WB-CAL-1), which was derived from NZW x BXSB F1 mouse (a model of antiphospholipid syndrome), significantly increased the oxLDL/beta(2)GPI binding to macrophages. In contrast, IgM anti-oxLDL natural antibody, EO6 (derived from apoe( -/-) mouse), prevented the binding. Different antigenic specificity of these antibodies to oxLDL and its beta(2)GPI complexes was also confirmed in TLC-ligand blot and ELISA. Thus, IgG anti-beta(2) GPI autoantibodies contribute to lipid metabolism (housekeeping of oxLDL by macrophages) whereas IgM natural anti-oxLDL antibodies may protect against atherogenesis. In addition, in vitro data suggest that relatively high dose of intravenous immunoglobulin preparations (mainly contain IgG anti-oxLDL antibodies) might also prevent atherogenesis by inhibiting the oxLDL binding to macrophages.
|
| Volume |
16(12)
|
| Pages |
929-38
|
| Published |
2007-1-1
|
| DOI |
10.1177/0961203307084170
|
| PII |
16/12/929
|
| PMID |
18042586
|
| MeSH |
Animals
Antibodies, Antiphospholipid / physiology*
Antibody Specificity
Antiphospholipid Syndrome / immunology*
Atherosclerosis / immunology*
Cell Line
Humans
Immunoglobulin G / immunology
Immunoglobulin G / physiology
Immunoglobulin M / immunology
Immunoglobulin M / physiology
Immunoglobulins, Intravenous / immunology
Lipoproteins, LDL / immunology*
Lipoproteins, LDL / metabolism
Macrophages / immunology
Macrophages / physiology*
Mice
beta 2-Glycoprotein I / physiology*
|
| IF |
2.251
|
| Times Cited |
26
|
|
WOS Category
|
RHEUMATOLOGY
|
| Resource |
| Human and Animal Cells |
J774.1(RCB0434) |
