| RRC ID |
43188
|
| 著者 |
Tanimoto Y, Shibata Y, Kataoka Y, Miyazaki T, Nishiyama N.
|
| タイトル |
Osteoblast-like cell proliferation on tape-cast and sintered tricalcium phosphate sheets.
|
| ジャーナル |
Acta Biomater
|
| Abstract |
The influence of sintering temperature on the in vitro proliferation of osteoblast-like cells to sintered tricalcium phosphate (TCP) sheets prepared by tape-casting was investigated. Green sheets of tape-cast beta-TCP were sintered for 2h in a furnace at atmospheric pressure at five different sintering temperatures: 900, 1000, 1100, 1150 and 1200 degrees C. The number of osteoblast-like (MC3T3-E1) cells deposited onto TCP sheets was counted after cell cultivation for 1week and was found to have increased with increasing sintering temperature. The TCP sheets sintered at 900 degrees C exhibited a significantly lower cell number than TCP sheets sintered at 1000, 1100, 1150 and 1200 degrees C. In the attenuated total reflection infrared spectra, the peaks around 900-1150cm(-1), corresponding to the P-O vibration mode of the phosphate group, gradually decreased and shifted to lower wavenumbers with increasing sintering temperature. Meanwhile, the zeta potential of TCP sintered at 900 degrees C showed a highly negative charge when compared with the other groups. This would suggest that the higher solubility of the TCP sheets sintered at 900 degrees C exerted the higher negative charge obtained from zeta potential measurement. Within the limitations of this study, it was indicated that osteoblast-like cell proliferation increased with increasing sintering temperature. The biological stability of the sintered TCP sheet surface was considered to have affected cell proliferation.
|
| 巻・号 |
4(2)
|
| ページ |
397-402
|
| 公開日 |
2008-3-1
|
| DOI |
10.1016/j.actbio.2007.10.001
|
| PII |
S1742-7061(07)00165-1
|
| PMID |
18054299
|
| MeSH |
3T3 Cells
Animals
Biocompatible Materials* / chemistry
Calcium Phosphates* / chemistry
Cell Proliferation
Hot Temperature
Materials Testing
Mice
Osteoblasts / cytology*
Spectrophotometry, Infrared
X-Ray Diffraction
|
| IF |
7.242
|
| 引用数 |
14
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
