論文 - 詳細
| RRC ID | 43189 |
|---|---|
| 著者 | Yamada Y, Akita H, Kamiya H, Kogure K, Yamamoto T, Shinohara Y, Yamashita K, Kobayashi H, Kikuchi H, Harashima H. |
| タイトル | MITO-Porter: A liposome-based carrier system for delivery of macromolecules into mitochondria via membrane fusion. |
| ジャーナル | Biochim Biophys Acta |
| Abstract |
Mitochondria are the principal producers of energy in higher cells. Mitochondrial dysfunction is implicated in a variety of human diseases, including cancer and neurodegenerative disorders. Effective medical therapies for such diseases will ultimately require targeted delivery of therapeutic proteins or nucleic acids to the mitochondria, which will be achieved through innovations in the nanotechnology of intracellular trafficking. Here we describe a liposome-based carrier that delivers its macromolecular cargo to the mitochondrial interior via membrane fusion. These liposome particles, which we call MITO-Porters, carry octaarginine surface modifications to stimulate their entry into cells as intact vesicles (via macropinocytosis). We identified lipid compositions for the MITO-Porter which promote both its fusion with the mitochondrial membrane and the release of its cargo to the intra-mitochondrial compartment in living cells. Thus, the MITO-Porter holds promise as an efficacious system for the delivery of both large and small therapeutic molecules into mitochondria. |
| 巻・号 | 1778(2) |
| ページ | 423-32 |
| 公開日 | 2008-2-1 |
| DOI | 10.1016/j.bbamem.2007.11.002 |
| PII | S0005-2736(07)00429-4 |
| PMID | 18054323 |
| MeSH | Animals HeLa Cells Humans Liposomes* Male Membrane Fusion* Microscopy, Fluorescence Mitochondria, Liver / metabolism* Rats Rats, Wistar |
| IF | 3.411 |
| 引用数 | 155 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |