論文 - 詳細
| RRC ID | 43228 |
|---|---|
| 著者 | Maekawa K, Yoshida Y, Mine A, van Meerbeek B, Suzuki K, Kuboki T. |
| タイトル | Effect of polyphosphoric acid pre-treatment of titanium on attachment, proliferation, and differentiation of osteoblast-like cells (MC3T3-E1). |
| ジャーナル | Clin Oral Implants Res |
| Abstract |
PURPOSE:To evaluate the effect of polyphosphoric acid (PPA) pre-treatment of titanium (Ti) on the initial attachment, proliferation, and differentiation of mouse osteoblast-like cells (MC3T3-E1). MATERIALS AND METHODS:Adsorption of PPA to Ti was achieved by immersing Ti disks (15 mm in diameter) into 0, 1, and 10 wt% PPA and 10 wt% orthophosphoric acid (OPA) for 24 h. On each pre-treated Ti disk, 5.0 x 10(4) MC3T3-E1 cells were seeded, and 1, 3, and 5 h later cell attachment was evaluated. Cell proliferation was also determined 1, 3, and 5 days after cell seed. Cell differentiation was evaluated 5, 10, and 15 days after cell seed using osteoblast marker gene expression. Total RNA was purified from each culture and Type-I collagen, alkaline phosphatase, and osteocalcin mRNA expression levels were measured by real-time reverse transcription polymerase chain reaction. RESULTS:Adsorption of PPA or OPA to Ti significantly accelerated initial cell attachment at every time point (P<0.0001). As with cell attachment, cell proliferation was also accelerated on the PPA-treated Ti disks in a dose-dependent manner at every time point (P<0.0001). However, OPA-treated Ti disks did not enhance the cell proliferation. Regarding osteoblastic differentiation, PPA-treated Ti significantly accelerated the Type-I collagen gene expression at 5 and 10 days after cell seed. Regarding alkaline phosphatase and osteocalcin gene expression, no significant difference was found among the different Ti surface conditions. CONCLUSION:The accelerated cell behavior following Ti pre-treatment with PPA is a promising new strategy to fabricate bioactive Ti implants. |
| 巻・号 | 19(3) |
| ページ | 320-5 |
| 公開日 | 2008-3-1 |
| DOI | 10.1111/j.1600-0501.2007.01477.x |
| PII | CLR1477 |
| PMID | 18190561 |
| MeSH | 3T3 Cells Acid Etching, Dental* Alkaline Phosphatase / biosynthesis Analysis of Variance Animals Cell Adhesion Cell Differentiation Cell Proliferation Collagen Type I / biosynthesis Gene Expression Mice Osteoblasts / metabolism Osteoblasts / physiology* Osteocalcin / biosynthesis Phosphoric Acids / pharmacology* Polymers / pharmacology* RNA, Messenger / biosynthesis Reverse Transcriptase Polymerase Chain Reaction Surface Properties Titanium* |
| IF | 3.723 |
| 引用数 | 12 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE ENGINEERING, BIOMEDICAL |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |