論文 - 詳細
| RRC ID | 43232 |
|---|---|
| 著者 | Kajikawa H, Yoshida N, Katada K, Hirayama F, Handa O, Kokura S, Naito Y, Yoshikawa T. |
| タイトル | Helicobacter pylori activates gastric epithelial cells to produce interleukin-8 via protease-activated receptor 2. |
| ジャーナル | Digestion |
| Abstract |
BACKGROUND/AIM:Recently, it has been shown that serine proteases derived from microorganisms stimulate epithelial cells to produce inflammatory mediators through protease-activated receptor (PAR). We investigated the involvement of PAR2 in the interleukin (IL)-8 production by Helicobacter pylori-infected gastric epithelial cells. METHODS AND RESULTS:Human gastric epithelial cells, MKN45 cells, were used. The expression of PAR2 was assessed by real-time PCR and immunocytochemistry, and IL-8 protein was measured by an enzyme-linked immunosorbent assay. PAR2 mRNA and protein were constitutively expressed on unstimulated MKN45 cells. The treatment of cells with H. pylori resulted in a significant increase in PAR2 expression. In addition, trypsin (a natural PAR2 agonist), SLIGKV amide (a synthetic PAR2 agonist), H. pylori live bacteria or H. pylori culture supernatant significantly induced IL-8 production from MKN45 cells. H. pylori-induced IL-8 production was inhibited by nafamostat mesilate (a serine protease inhibitor), neutralizing antibody to PAR2 and in PAR2-deficient cells treated with siRNA. CONCLUSIONS:These results reveal that H. pylori-derived protease activates gastric epithelial cells to produce inflammatory cytokines through PAR2, suggesting an important role for PAR2 in the modulation of gastric inflammation associated with H. pylori. |
| 巻・号 | 76(3-4) |
| ページ | 248-55 |
| 公開日 | 2007-1-1 |
| DOI | 10.1159/000113041 |
| PII | 000113041 |
| PMID | 18196899 |
| MeSH | Antibodies / pharmacology Benzamidines Cell Line, Tumor Epithelial Cells / metabolism* Epithelial Cells / microbiology Gastric Mucosa / metabolism* Gastric Mucosa / microbiology Gene Expression Guanidines / pharmacology Helicobacter Infections / metabolism* Helicobacter pylori / enzymology* Humans Interleukin-8 / metabolism* RNA, Small Interfering / pharmacology Receptor, PAR-2 / agonists Receptor, PAR-2 / genetics Receptor, PAR-2 / immunology Receptor, PAR-2 / metabolism* Serine Proteinase Inhibitors / pharmacology Transfection |
| IF | 2.692 |
| 引用数 | 24 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |