| RRC ID |
43258
|
| 著者 |
Tanne Y, Tanimoto K, Tanaka N, Ueki M, Lin YY, Ohkuma S, Kamiya T, Tanaka E, Tanne K.
|
| タイトル |
Expression and activity of Runx2 mediated by hyaluronan during chondrocyte differentiation.
|
| ジャーナル |
Arch Oral Biol
|
| Abstract |
During endochondral ossification, the production of hyaluronan (HA) is strictly and selectively regulated by chondrocytes, with a temporal peak at the hypertrophic stage. This study was conducted to clarify the effects of HA on expression and activity of runt-related gene 2 (Runx2), a potent transcription factor for chondrocyte differentiation in hypertrophic chondrocytes. Immature chondrocytes from an ATDC5 cell line were cultured and differentiated in DMEM/Ham's F12 with pre-defined supplements. Using real-time PCR, the gene expressions of type II collagen, MMP-13, HAS2, and Runx2 in cultured chondrocytes were analysed from days 0 to 18 of cell differentiation. The activity and expression of Runx2 in hypertrophic chondrocytes were analysed after the treatment with HA oligosaccharide (HAoligo) using AML-3/Runx2 binding, real-time PCR and Western blot analysis. The effects of pre-incubation of anti-CD44 antibody on Runx2 expression were also examined. Expression of type X collagen and Runx2 mRNAs reached a maximum at the terminal differentiation of chondrocytes. The activity and expression of Runx2 was significantly inhibited in hypertrophic chondrocytes treated with HAoligo compared to the untreated controls. High molecular weight-HA did not affect the expression or activity of Runx2. The expression of Runx2 mRNA was significantly decreased in hypertrophic chondrocytes treated with anti-CD44 antibody. These results suggest that HAoligo may affect the terminal differentiation of chondrocytes during the endochondral ossification by inhibiting the expression and activity of Runx2.
|
| 巻・号 |
53(5)
|
| ページ |
478-87
|
| 公開日 |
2008-5-1
|
| DOI |
10.1016/j.archoralbio.2007.12.007
|
| PII |
S0003-9969(07)00313-5
|
| PMID |
18242579
|
| MeSH |
Animals
Blotting, Western
Cell Differentiation / drug effects*
Cell Differentiation / physiology
Cell Line, Tumor / drug effects
Cell Line, Tumor / physiology
Chondrocytes / cytology
Chondrocytes / drug effects*
Chondrocytes / metabolism
Collagen Type II / genetics
Collagen Type II / metabolism
Collagen Type X / genetics
Collagen Type X / metabolism
Core Binding Factor Alpha 1 Subunit / genetics
Core Binding Factor Alpha 1 Subunit / metabolism*
Gene Expression
Glucuronosyltransferase / genetics
Glucuronosyltransferase / metabolism
Hyaluronan Receptors / metabolism
Hyaluronan Synthases
Hyaluronic Acid / pharmacology*
Matrix Metalloproteinase 13 / genetics
Matrix Metalloproteinase 13 / metabolism
Mice
RNA / metabolism
RNA, Mitochondrial
Reverse Transcriptase Polymerase Chain Reaction
Transcription Factors / genetics
Transcription Factors / metabolism
|
| IF |
1.931
|
| 引用数 |
12
|
|
WOS 分野
|
DENTISTRY, ORAL SURGERY & MEDICINE
|
| リソース情報 |
| ヒト・動物細胞 |
|
