RRC ID 43259
Author Sugiura S, Edahiro J, Sumaru K, Kanamori T.
Title Surface modification of polydimethylsiloxane with photo-grafted poly(ethylene glycol) for micropatterned protein adsorption and cell adhesion.
Journal Colloids Surf B Biointerfaces
Abstract In this study, we applied photo-induced graft polymerization to micropatterned surface modification of polydimethylsiloxane (PDMS) with poly(ethylene glycol). Two types of monomers, polyethylene glycol monoacrylate (PEGMA) and polyethylene glycol diacrylate (PEGDA), were tested for surface modification of PDMS. Changes in the surface hydrophilicity and surface element composition were characterized by contact angle measurement and electron spectroscopy for chemical analysis. The PEGMA-grafted PDMS surfaces gradually lost their hydrophilicity within two weeks. In contrast, the PEGDA-grafted PDMS surface maintained stable hydrophilic characteristics for more than two months. Micropatterned protein adsorption and micropatterned cell adhesion were successfully demonstrated using PEGDA-micropatterned PDMS surfaces, which were prepared by photo-induced graft polymerization using photomasks. The PEGDA-grafted PDMS exhibited useful characteristics for microfluidic devices (e.g. hydrophilicity, low protein adsorption, and low cell attachment). The technique presented in this study will be useful for surface modification of various research tools and devices.
Volume 63(2)
Pages 301-5
Published 2008-6-1
DOI 10.1016/j.colsurfb.2007.12.013
PII S0927-7765(07)00488-2
PMID 18242961
MeSH Adsorption Cell Adhesion* Cell Line Dimethylpolysiloxanes / chemistry* Humans Photochemistry Polyethylene Glycols / chemistry* Proteins / chemistry* Surface Properties
IF 3.973
Times Cited 72
WOS Category BIOPHYSICS CHEMISTRY, PHYSICAL MATERIALS SCIENCE, BIOMATERIALS
Resource
Human and Animal Cells