| RRC ID |
43262
|
| Author |
Muto N, Tomokuni T, Haramoto M, Tatemoto H, Nakanishi T, Inatomi Y, Murata H, Inada A.
|
| Title |
Isolation of apoptosis- and differentiation-inducing substances toward human promyelocytic leukemia HL-60 cells from leaves of Juniperus taxifolia.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
A chloroform extract of the leaves of Juniperas taxifolia exhibited a marked antiproliferative effect on human promyelocytic leukemia HL-60 cells at a concentration of 2.5 microg/ml. Deoxypodophyllotoxin (4) was identified in the extract as an outstanding antiproliferative compound, and five diterpenes (1-3, 5, and 6) were isolated as known compounds with weak or no cytotoxicity. These compounds were examined for their respective apoptosis- and differentiation-inducing activities toward HL-60 cells by DNA fragmentation and NBT-reducing assays, respectively. Among them, 7alpha-hydroxysandaracopimaric acid (6) was found to have a potent differentiation-inducing activity in a dose-dependent manner at 0.125-2 microg/ml (0.39-6.29 microM), together with apoptosis-inducing activity at concentrations of more than 2.5 microg/ml (7.86 microM). Deoxypodophyllotoxin (4) that exerted cytotoxic and apoptosis-inducing activities at 2 ng/ml (5 nM) did not induce differentiation at the same concentration, and the other diterpenes (1-3 and 5) showed no effect on cell differentiation, even at 5 microg/ml. It was thus demonstrated for the first time that 7alpha-hydroxysandaracopimaric acid was an effective differentiation-inducing compound toward HL-60 cells.
|
| Volume |
72(2)
|
| Pages |
477-84
|
| Published |
2008-2-1
|
| DOI |
10.1271/bbb.70570
|
| PII |
JST.JSTAGE/bbb/70570
|
| PMID |
18256464
|
| MeSH |
Apoptosis / drug effects*
Cell Differentiation / drug effects*
HL-60 Cells
Humans
Juniperus / chemistry*
Leukemia, Promyelocytic, Acute / pathology*
Magnetic Resonance Spectroscopy
Plant Extracts / pharmacology*
Plant Leaves / chemistry*
Spectrometry, Mass, Electrospray Ionization
|
| IF |
1.516
|
| Times Cited |
42
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
HL60(RCB0041) |
