Reference - Detail
| RRC ID | 43262 |
|---|---|
| Author | Muto N, Tomokuni T, Haramoto M, Tatemoto H, Nakanishi T, Inatomi Y, Murata H, Inada A. |
| Title | Isolation of apoptosis- and differentiation-inducing substances toward human promyelocytic leukemia HL-60 cells from leaves of Juniperus taxifolia. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
A chloroform extract of the leaves of Juniperas taxifolia exhibited a marked antiproliferative effect on human promyelocytic leukemia HL-60 cells at a concentration of 2.5 microg/ml. Deoxypodophyllotoxin (4) was identified in the extract as an outstanding antiproliferative compound, and five diterpenes (1-3, 5, and 6) were isolated as known compounds with weak or no cytotoxicity. These compounds were examined for their respective apoptosis- and differentiation-inducing activities toward HL-60 cells by DNA fragmentation and NBT-reducing assays, respectively. Among them, 7alpha-hydroxysandaracopimaric acid (6) was found to have a potent differentiation-inducing activity in a dose-dependent manner at 0.125-2 microg/ml (0.39-6.29 microM), together with apoptosis-inducing activity at concentrations of more than 2.5 microg/ml (7.86 microM). Deoxypodophyllotoxin (4) that exerted cytotoxic and apoptosis-inducing activities at 2 ng/ml (5 nM) did not induce differentiation at the same concentration, and the other diterpenes (1-3 and 5) showed no effect on cell differentiation, even at 5 microg/ml. It was thus demonstrated for the first time that 7alpha-hydroxysandaracopimaric acid was an effective differentiation-inducing compound toward HL-60 cells. |
| Volume | 72(2) |
| Pages | 477-84 |
| Published | 2008-2-1 |
| DOI | 10.1271/bbb.70570 |
| PII | JST.JSTAGE/bbb/70570 |
| PMID | 18256464 |
| MeSH | Apoptosis / drug effects* Cell Differentiation / drug effects* HL-60 Cells Humans Juniperus / chemistry* Leukemia, Promyelocytic, Acute / pathology* Magnetic Resonance Spectroscopy Plant Extracts / pharmacology* Plant Leaves / chemistry* Spectrometry, Mass, Electrospray Ionization |
| IF | 1.516 |
| Times Cited | 42 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HL60(RCB0041) |